CAJ | 학술논문

目的探索建立犬月骨缺血性坏死动物实验模型的方法.方法成年健康杂种犬12只,通过剥离左侧月骨周围组织及液氮冷冻的方法致其缺血坏死,分别在术后第3、6、12周动态地观察其影像学(X线片、CT及MBI)表现,并取月骨进行大体标本及组织形态学检查.结果第3周时X线片及CT检查没有明显的影像学改变,MRI检查T2WI上表现为弥漫性的点状高信号影.第6周时X线片及CT检查没有明显的影像学改变；MRI表现异常,T2WI上表现为片状高信号影.第12周时X线片示腕骨骨密度不均匀,腕骨外形不规则,压缩变扁；CT示大面积不规则的低密度灶；MRI检查T2WI上见腕骨内部大面积的高信号影；此时大体标本见大片白色死骨,关节软骨脱落；光镜下见骨小梁密度减低,大量骨陷窝形成.结论运用液氮冷冻并血管破坏可以制备典型的犬月骨缺血性坏死动物实验模型.
목적 탐색건립견월골결혈성배사동물실험모형적방법.방법 성년건강잡충견12지,통과박리좌측월골주위조직급액담냉동적방법치기결혈배사,분별재술후제3、6、12주동태지관찰기영상학(X선편、CT급MBI)표현,병취월골진행대체표본급조직형태학검사.결과 제3주시X선편급CT검사몰유명현적영상학개변,MRI검사T2WI상표현위미만성적점상고신호영.제6주시X선편급CT검사몰유명현적영상학개변；MRI표현이상,T2WI상표현위편상고신호영.제12주시X선편시완골골밀도불균균,완골외형불규칙,압축변편；CT시대면적불규칙적저밀도조；MRI검사T2WI상견완골내부대면적적고신호영；차시대체표본견대편백색사골,관절연골탈락；광경하견골소량밀도감저,대량골함와형성.결론 운용액담냉동병혈관파배가이제비전형적견월골결혈성배사동물실험모형.
Objective To establish a canine model for the study of ischemic necrosis of the lunate.Methods Twelve adult canines were used.The lunate was exposed and stripped off its peripheral tissues and then frozen by liquid nitrogen.X-ray,CT and MRI images of the lunate were acquired at 3,6 and 12 weeks to track the dynamic changes.Lunate was harvested at these time points for gross and histology examination to evaluate the necrosis of lunate and subsequent repairing reaction.Results At 3 weeks there were no significant changes on X-ray and CT.Diffused sporadic enhanced signals were seen on T2 weighted MRI images.At 6 weeks X-ray and CT scans were still insignificant.However T2 weighted MRI showed more abnormality with areas of enhanced signals.At 12 weeks heterogenous bone density,irregularity and compression deformity of the carpal bone were seen on X-ray.CT scans showed large areas of low density loci,while MRI showed large areas of enhanced signals on T2 weighted images.There were significant changes in gross and microscopic manifestation of the lunate.Large area of whitish necrotic bone and decaying of cartilage surface were seen.Light microscopic sections revealed decreased bone density in the trabeculum and loci of bone collapse.Conclusion Liquid nitrogen freezing and devascularzation of the lunate is a successful and reliable method to establish a canine model of Kienbock's disease.