CAJ | 학술논문

一氧化二氮还原酶是堆肥发酵中反硝化菌代表性的酶，可较准确地反映出堆肥中反硝化菌的活动状态及反硝化作用状态。文章采用PCR-DGGE方法研究堆肥中反硝化菌群落的变化，结果表明，添加菌剂的堆肥（堆肥Ⅰ和Ⅱ）中反硝化菌的数量与种类都有所增加（尤其是在堆肥Ⅱ中）。通过对DGGE图谱上的条带进行克隆测序，发现测序条带多为假单胞菌。
일양화이담환원매시퇴비발효중반초화균대표성적매，가교준학지반영출퇴비중반초화균적활동상태급반초화작용상태。문장채용PCR-DGGE방법연구퇴비중반초화균군락적변화，결과표명，첨가균제적퇴비（퇴비Ⅰ화Ⅱ）중반초화균적수량여충류도유소증가（우기시재퇴비Ⅱ중）。통과대DGGE도보상적조대진행극륭측서，발현측서조대다위가단포균。
Nitrous oxide reductase is the representative of denitrifying bacteria accurately reflecting the state of the organisms and denitrification in compost. In this study, PCR-DGGE was used to study the structure and diversity of nitrous oxide reductase bacteria community in compost. The results showed that the number and species of denitrifying bacteria increased in compost (compost Ⅰ and Ⅱ, especial y in compost Ⅱ) inoculated with exogenous organisms. The cloning and sequencing of the DGGE bands revealed that they were affiliated to Pseudomonas.