东北农业大学学报
東北農業大學學報
동북농업대학학보
JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY
2014年
3期
34-39
,共6页
姜景彬%彭祥珍%许向阳%陈秀玲%李景富
薑景彬%彭祥珍%許嚮暘%陳秀玲%李景富
강경빈%팽상진%허향양%진수령%리경부
番茄黄化曲叶病毒病%Ty-1%遗传分析%AFLP
番茄黃化麯葉病毒病%Ty-1%遺傳分析%AFLP
번가황화곡협병독병%Ty-1%유전분석%AFLP
tomato yel ow leaf curl virus disease%Ty-1%genetic analysis%AFLP
研究以番茄黄化曲叶病抗病品种“10033”与感病品种“10981”为亲本配制杂交组合，利用P1、P2、F1、F2、BC1P1和BC1P26个世代进行番茄黄化曲叶病抗性基因Ty-1的遗传规律分析。用288对AFLP引物对215株F2代分离群体进行连锁分析。得到4个与番茄黄化曲叶病抗病基因Ty-1连锁的AFLP标记分别是E62M37-D、E51M 44-C、E45M61-E、E40M60-A，与抗病基因Ty-1的连锁遗传距离分别为8.9、17.5、19.6和30.8 cM。
研究以番茄黃化麯葉病抗病品種“10033”與感病品種“10981”為親本配製雜交組閤，利用P1、P2、F1、F2、BC1P1和BC1P26箇世代進行番茄黃化麯葉病抗性基因Ty-1的遺傳規律分析。用288對AFLP引物對215株F2代分離群體進行連鎖分析。得到4箇與番茄黃化麯葉病抗病基因Ty-1連鎖的AFLP標記分彆是E62M37-D、E51M 44-C、E45M61-E、E40M60-A，與抗病基因Ty-1的連鎖遺傳距離分彆為8.9、17.5、19.6和30.8 cM。
연구이번가황화곡협병항병품충“10033”여감병품충“10981”위친본배제잡교조합，이용P1、P2、F1、F2、BC1P1화BC1P26개세대진행번가황화곡협병항성기인Ty-1적유전규률분석。용288대AFLP인물대215주F2대분리군체진행련쇄분석。득도4개여번가황화곡협병항병기인Ty-1련쇄적AFLP표기분별시E62M37-D、E51M 44-C、E45M61-E、E40M60-A，여항병기인Ty-1적련쇄유전거리분별위8.9、17.5、19.6화30.8 cM。
In this study, crosses were made with resistant tomato variety 10033 and susceptible variety 10981 to tomato yellow leaf curl virus disease. Six generation P1, P2, F1, F2, BC1P1 and BC1P2 were used to analyze the genetic of Ty-1. Through AFLP analysis of 215 F2 progenies using 288 AFLP primer combinations. Four AFLP markers linked to the Ty-1 were identified. The genetic distance of E62M37-D, E51M44-C, E45M61-E and E40M60-A were 8.9, 17.5, 19.6 and 30.8 cM, respectively.
