CAJ | 학술논문

目的：探讨TGF-β1、Smad7与ki-67 mRNA在胃癌中的表达及其相关性。方法：手术切除经病理证实为胃癌组织标本60例，正常胃组织20例，采用RT-PCR技术检测TGF-β1 mRNA、Smad7 mRNA和ki-67 mRNA表达水平。结果：TGF-β1、Smad7和ki-67 mRNA在正常胃组织中的相对含量均低于胃癌组织中的表达（P＜0．05）；三者在高中分化胃癌组织的相对含量均低于低分化胃癌组织（P＜0．05）；TGF-β1 mRNA、Smad7 mRNA和ki-67 mRNA在无淋巴结转移者的表达低于有淋巴结转移者；TNM分期：Ⅰ＋Ⅱ期的表达低于Ⅲ＋Ⅳ期（P＜0．05）；TGF-β1 mRNA、Smad7 mRNA、ki-67 mRNA三者在胃癌中的表达经直线相关分析显示呈正相关。结论：TGF-β1、Smad7与ki-67 mRNA的表达与胃癌的发生、发展及分化程度密切相关。
목적：탐토TGF-β1、Smad7여ki-67 mRNA재위암중적표체급기상관성。방법：수술절제경병리증실위위암조직표본60례，정상위조직20례，채용RT-PCR기술검측TGF-β1 mRNA、Smad7 mRNA화ki-67 mRNA표체수평。결과：TGF-β1、Smad7화ki-67 mRNA재정상위조직중적상대함량균저우위암조직중적표체（P＜0．05）；삼자재고중분화위암조직적상대함량균저우저분화위암조직（P＜0．05）；TGF-β1 mRNA、Smad7 mRNA화ki-67 mRNA재무림파결전이자적표체저우유림파결전이자；TNM분기：Ⅰ＋Ⅱ기적표체저우Ⅲ＋Ⅳ기（P＜0．05）；TGF-β1 mRNA、Smad7 mRNA、ki-67 mRNA삼자재위암중적표체경직선상관분석현시정정상관。결론：TGF-β1、Smad7여ki-67 mRNA적표체여위암적발생、발전급분화정도밀절상관。
Objective To investigate relationship between the expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA and the clinicopathologic characteristics in gastric carcinoma. Methods 60 cases with gastric carcinoma tissue and 20 cases with normal gastric tissue, which were surgically removed and certified by pathology, were collected. The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA were detected by RT-PCR in normal and carcinomatous gastric tissue. Results The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in normal gastric tissue were lower than those in carcinomatous gastric tissue (P < 0.05). The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in gastric carcinoma which were well differentiated and moderately differentiated were significantly lower than those in poorly differentiated gastric carcinoma (P<0.05). The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in gastric carcinoma without lymph node metastasis were lower than those in gastric carcinoma with lymph node metastasis. The expressions of TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in Ⅰ+Ⅱ stage were lower those in Ⅲ+Ⅳ stage (P < 0.05). Linear correlation showed there were positive correlations among TGF-β1 mRNA, Smad7 mRNA and ki-67 mRNA in gastric carcinoma. Conclusions The canonical TGF-β1 Smad7 and Ki-67 mRNA play a role in the carcinogenesis, development, differentiation of gastric carcinoma.