CAJ | 학술논문

利用固定波长荧光分光光度法（Fixed-wavelength fluorescence，简称 FF 法）研究了苯并（a）芘暴露下罗非鱼胆汁代谢物的动态变化。在试验中首先优化了 FF 法测定罗非鱼胆汁代谢物的稀释倍数，将罗非鱼胆汁分别稀释1000、2000、4000、8000倍，在激发/散发波长380 nm/430 nm 测定荧光强度。结果显示2000倍左右的稀释较为合理，结合其他研究，试验最终采用1600倍的稀释倍数。在此基础上利用优化后的 FF 法研究了不同浓度的 BaP（0.1、1、10、50μg·L-1）暴露下罗非鱼胆汁代谢物的动态变化。结果显示：0.1μg·L-1浓度组的 BaP 代谢物随时间变化无明显波动，与对照组无显著差异（P﹥0.05）；其他3个剂量组从2 h 起就与对照组差异显著（P﹤0.05），3个试验组均呈现先上升后下降的趋势。胆汁中 BaP 代谢物随浓度升高不断升高，呈现明显的剂量-效应关系，表明鱼体内胆汁代谢物可用来反映周围水环境中 BaP 的污染情况。这为将 FF 法应用于环境中 BaP 的生物监测，使胆汁代谢物成为BaP 环境监测的有效生物标志物提供了理论依据。
이용고정파장형광분광광도법（Fixed-wavelength fluorescence，간칭 FF 법）연구료분병（a）비폭로하라비어담즙대사물적동태변화。재시험중수선우화료 FF 법측정라비어담즙대사물적희석배수，장라비어담즙분별희석1000、2000、4000、8000배，재격발/산발파장380 nm/430 nm 측정형광강도。결과현시2000배좌우적희석교위합리，결합기타연구，시험최종채용1600배적희석배수。재차기출상이용우화후적 FF 법연구료불동농도적 BaP（0.1、1、10、50μg·L-1）폭로하라비어담즙대사물적동태변화。결과현시：0.1μg·L-1농도조적 BaP 대사물수시간변화무명현파동，여대조조무현저차이（P﹥0.05）；기타3개제량조종2 h 기취여대조조차이현저（P﹤0.05），3개시험조균정현선상승후하강적추세。담즙중 BaP 대사물수농도승고불단승고，정현명현적제량-효응관계，표명어체내담즙대사물가용래반영주위수배경중 BaP 적오염정황。저위장 FF 법응용우배경중 BaP 적생물감측，사담즙대사물성위BaP 배경감측적유효생물표지물제공료이론의거。
Biomarker plays an important role in environmental pollution monitoring. Here dynamics of benzo(a)pyrene(BaP)metabolites in tilapia bile were monitored under artificial exposure using fixed-wavelength fluorescence method(FF). Dilution rate of tilapia bile for the FF method was first developed by diluting bile at 1:1000, 1:2000, 1:4000, and 1:8000 and setting excitation/emission wavelength of spectrofluo-rophotometer at 380 nm/430 nm. Dilution rate at 2000-fold was found to be reasonable. Combined with other studies, 1600-fold dilution was selected in the present experiment to examine the dose and time effects on dynamics of BaP metabolites in bile (0.1 μg·L-1, 1 μg·L-1, 10 μg·L-1 and 50 μg·L-1). At 0.1 μg BaP·L-1, BaP metabolites neither showed obvious fluctuation nor had significantly differences from the control group(P﹥0.05). However, significant differences were observed between BaP metabolites at the other three doses and the control, be-ginning from 2 h(P﹤0.05). The fluorescence intensity showed“rise then fall”trend at concentrations of 1 μg·L-1, 10 μg·L-1, and 50 μg·L-1. The metabolites of BaP in bile had a significant dose-response relationship. These results demonstrate that BaP dynamics in tilapia bile are certainly sensitive biomarkers to assess fish exposure to BaP and FF method can be widely used in the environment monitoring.