CAJ | 학술논문

通过液体摇床振荡培养实验，获得了链孢囊菌降解纤维素形成的菌体，采用元素组成、差热分析和红外光谱法，初步研究其化学结构和碱提取组分碳的分配状况，旨在探索链孢囊菌降解纤维素后是否能形成腐殖质及其碱提取组分是否为腐殖质组分。结果表明：（1）链孢囊菌降解纤维素后形成的菌体各组分碳量的分配主要集中于“水溶性组分”与“碱溶酸不溶组分”（相当于类胡敏酸），“碱溶酸溶组分”（相当于类富里酸）最少，培养后期（38~60 d）各组分碳，随着培养时间的延长，先形成“碱溶酸溶组分”后转化为“碱溶酸不溶组分”的速率减慢；（2）接种链孢囊菌后，培养初期（7~28 d），菌体 C 含量、H/C、O﹢S/C、放热高/中比，均呈现不规律的动态变化，培养后期（38~60 d），随着培养时间的延长，菌体缩合程度升高，氧化程度降低，芳香性增强；（3）菌体（60 d）的 H/C、O﹢S/C均高于黑土胡敏酸（HA），而菌体的 C 含量、放热高/中比均低于黑土胡敏酸（HA），且分子结构较黑土胡敏酸（HA）简单。
통과액체요상진탕배양실험，획득료련포낭균강해섬유소형성적균체，채용원소조성、차열분석화홍외광보법，초보연구기화학결구화감제취조분탄적분배상황，지재탐색련포낭균강해섬유소후시부능형성부식질급기감제취조분시부위부식질조분。결과표명：（1）련포낭균강해섬유소후형성적균체각조분탄량적분배주요집중우“수용성조분”여“감용산불용조분”（상당우류호민산），“감용산용조분”（상당우류부리산）최소，배양후기（38~60 d）각조분탄，수착배양시간적연장，선형성“감용산용조분”후전화위“감용산불용조분”적속솔감만；（2）접충련포낭균후，배양초기（7~28 d），균체 C 함량、H/C、O﹢S/C、방열고/중비，균정현불규률적동태변화，배양후기（38~60 d），수착배양시간적연장，균체축합정도승고，양화정도강저，방향성증강；（3）균체（60 d）적 H/C、O﹢S/C균고우흑토호민산（HA），이균체적 C 함량、방열고/중비균저우흑토호민산（HA），차분자결구교흑토호민산（HA）간단。
It has been observed that Streptosporangium sp. grow on decomposing cellulous. In this study, using elementary analysis, differen-tial thermal analysis and infrared spectrometry, we examined the chemical structure of the mycelia of Streptosporangium sp. during cellulose decomposition. Carbon-containing components of alkali extraction from the mycelia were analyzed by modified humus composition method. The production rate of mycelia increased significantly with incubation time. The carbon in alkali extracts of the mycelia was mainly in “wa-ter-soluble”and“alkali-soluble but acid-insoluble(humic acid)”fractions. However, there was little carbon in“alkali soluble and acid soluble(fulvic acid)”fraction. During the 38th~60th day of incubation, C in“alkali soluble and acid soluble”and“residue(equivalent of humin)”increased, but the proportion of“alkali-soluble but acid-insoluble”to alkali-extractable fraction decreased gradually with incuba-tion time, indicating slowdown in formation of“alkali soluble but acid insoluble components”. At later stage of incubation(38~60 d), mycelia had reduced oxidation but increased condensation and aromaticity. The C/N, H/C, and O﹢S/C molar ratios of Streptsporangium sp. mycelia(60 d)were higher, but C content and exothermal high to medium ratios were lower than those of humic acid from black soil. The mycelia had relatively simple molecular structure as compared to humic acid from black soil. A longer incubation time is necessary to deter-mine whether during cellulose decomposition Streptosporangium could produce substances that have similar properties and structures to hu-mic acid from black soil.