农业环境科学学报
農業環境科學學報
농업배경과학학보
Journal of Agro-Environment Science
2014年
4期
680-686
,共7页
苏银萍%刘华%于方明%李艺%陈孟林%周振明%李明顺
囌銀萍%劉華%于方明%李藝%陳孟林%週振明%李明順
소은평%류화%우방명%리예%진맹림%주진명%리명순
Mn污染%木荷%抗氧化酶系统
Mn汙染%木荷%抗氧化酶繫統
Mn오염%목하%항양화매계통
manganese contamination%Schima superba%activity of antioxidant enzyme system
采用土培方法,研究了广西桂林市未受污染土壤(对照)及贺州市某锰矿的未开采区、探矿区、恢复区、采矿区土壤和尾砂对 Mn 富集植物木荷(Schima superba)生长、Mn 吸收,叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性,抗坏血酸(AsA)、谷胱甘肽(GSH)、植物络合素(PCs)、巯基(-SH)、丙二醛(MDA)、过氧化氢(H2O2)含量,以及超氧阴离子自由基(O-2·)产生速率的影响。结果表明,随着土壤 Mn 含量的增加,木荷根、茎、叶中 Mn 含量均显著增加(P﹤0.05),株高及株重呈先上升后下降的趋势,表明一定程度的 Mn 能促进木荷的生长。土壤 Mn 含量的增加,使得叶片中 O-2·的产生速率及H2O2的含量呈现不同程度的变化,采矿区土壤栽培的木荷叶片中 O-2·的产生速率显著高于对照(P﹤0.05),开采区土壤和尾砂栽培的木荷叶片中 H2O2含量比对照分别提高了65.7%、96.3%,矿区土壤栽培的木荷叶片中 MDA 的含量均显著高于对照(P﹤0.05),表明叶片受到一定的胁迫。木荷叶片中抗氧化酶活性与非酶物质变化不尽相同,SOD、POD 酶活性、AsA、GSH 含量均随着土壤 Mn 浓度的增加而增加,CAT、APX 酶的活性呈先升后降趋势,其中尾砂栽培的木荷 POD 酶的活性最高,比对照提高了1.62倍,采矿区土壤和尾砂栽培的木荷 GSH 的含量显著高于对照(P﹤0.05),分别比对照提高了1.66和1.79倍。
採用土培方法,研究瞭廣西桂林市未受汙染土壤(對照)及賀州市某錳礦的未開採區、探礦區、恢複區、採礦區土壤和尾砂對 Mn 富集植物木荷(Schima superba)生長、Mn 吸收,葉片超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、過氧化物酶(POD)、抗壞血痠過氧化物酶(APX)活性,抗壞血痠(AsA)、穀胱甘肽(GSH)、植物絡閤素(PCs)、巰基(-SH)、丙二醛(MDA)、過氧化氫(H2O2)含量,以及超氧陰離子自由基(O-2·)產生速率的影響。結果錶明,隨著土壤 Mn 含量的增加,木荷根、莖、葉中 Mn 含量均顯著增加(P﹤0.05),株高及株重呈先上升後下降的趨勢,錶明一定程度的 Mn 能促進木荷的生長。土壤 Mn 含量的增加,使得葉片中 O-2·的產生速率及H2O2的含量呈現不同程度的變化,採礦區土壤栽培的木荷葉片中 O-2·的產生速率顯著高于對照(P﹤0.05),開採區土壤和尾砂栽培的木荷葉片中 H2O2含量比對照分彆提高瞭65.7%、96.3%,礦區土壤栽培的木荷葉片中 MDA 的含量均顯著高于對照(P﹤0.05),錶明葉片受到一定的脅迫。木荷葉片中抗氧化酶活性與非酶物質變化不儘相同,SOD、POD 酶活性、AsA、GSH 含量均隨著土壤 Mn 濃度的增加而增加,CAT、APX 酶的活性呈先升後降趨勢,其中尾砂栽培的木荷 POD 酶的活性最高,比對照提高瞭1.62倍,採礦區土壤和尾砂栽培的木荷 GSH 的含量顯著高于對照(P﹤0.05),分彆比對照提高瞭1.66和1.79倍。
채용토배방법,연구료엄서계림시미수오염토양(대조)급하주시모맹광적미개채구、탐광구、회복구、채광구토양화미사대 Mn 부집식물목하(Schima superba)생장、Mn 흡수,협편초양화물기화매(SOD)、과양화경매(CAT)、과양화물매(POD)、항배혈산과양화물매(APX)활성,항배혈산(AsA)、곡광감태(GSH)、식물락합소(PCs)、구기(-SH)、병이철(MDA)、과양화경(H2O2)함량,이급초양음리자자유기(O-2·)산생속솔적영향。결과표명,수착토양 Mn 함량적증가,목하근、경、협중 Mn 함량균현저증가(P﹤0.05),주고급주중정선상승후하강적추세,표명일정정도적 Mn 능촉진목하적생장。토양 Mn 함량적증가,사득협편중 O-2·적산생속솔급H2O2적함량정현불동정도적변화,채광구토양재배적목하협편중 O-2·적산생속솔현저고우대조(P﹤0.05),개채구토양화미사재배적목하협편중 H2O2함량비대조분별제고료65.7%、96.3%,광구토양재배적목하협편중 MDA 적함량균현저고우대조(P﹤0.05),표명협편수도일정적협박。목하협편중항양화매활성여비매물질변화불진상동,SOD、POD 매활성、AsA、GSH 함량균수착토양 Mn 농도적증가이증가,CAT、APX 매적활성정선승후강추세,기중미사재배적목하 POD 매적활성최고,비대조제고료1.62배,채광구토양화미사재배적목하 GSH 적함량현저고우대조(P﹤0.05),분별비대조제고료1.66화1.79배。
Manganese(Mn)is essential for plant growth, but excess Mn may impact antioxidant enzyme systems in plant. A soil culture ex-periment was performed to explore the effects of Mn contamination on various enzymatic antioxidants and non-enzymatic antioxidants in the leaves of Schima superba. Soil samples, collected from unmining section(WK), prospecting area(T), restored area(H), mining(K)and tailings(W)in a manganese mine of Hezhou, were used as Mn-polluted soils, and an unpolluted soil from Guilin, Guangxi was used as con-trol(CK). After 145-d of culture, leaves of S. superba were sampled and analyzed for the activities of superoxide dismutase(SOD), peroxi-dase(POD), catalase(CAT), and ascorbate peroxidase(APX)and the contents of ascorbic acid(AsA), glutathione(GSH),phytochelatins (PCs),total acid soluble SH,malondialdehyde(MDA), hydrogen peroxide(H2O2), and the produce rate of superoxide anion(O-2·). Concen-trations of Mn in roots, stems, and leaves increased with soil Mn concentrations. Compared to the control, plant biomass increased in WK and T samples, whereas decreased in the other soils, indicating stimulation of S. superba growth by Mn at low concentrations. The rate of O-2· production in the leaves was significantly higher in H than in control(P﹤0.05). The contents of H2O2 in leaves were increased by 65.7% and 96.3% for K and W, respectively. The leaf MDA was significantly(P﹤0.05)higher in mineral soils than in control, showing that the leaves were damaged by Mn pollution. The activities or contents of SOD, POD, AsA and GSH increased in order of CK﹤WK﹤T﹤H﹤K﹤W, in con-sistency with soil Mn concentrations. The POD enzyme activity in leaves in W soil was 2.62 times as much as that of control. Both CAT and APX enzymatic activities increased in WK and T, but decreased in the others, as compared with the control. The contents of GSH in leaves in K and W were about 1.66 and 1.79 times higher than those of the control.