CAJ | 학술논문

为探索游离态 N-乙酰-β-D-氨基葡萄糖苷酶（简称 NAGase，又称壳二糖酶）作为农药对水生节肢动物胁迫效应指示因子的可行性，将大型溞、多刺裸腹溞、老年低额溞作为受试生物，虱螨脲、丁醚脲、乙螨唑作为受试药剂，研究了游离于水中的 NAGase的活性与溞类生物量之间的对应关系。以全部63个被暴露群体的生物量为自变量（x），以相应群体释放到水中的 NAGase 的活性为应变量（y），得到回归方程 y＝0.000603x﹢0.00433，该方程的 r 值和 P 值分别为0.687和0.00。两者之间的对应关系证明了利用水中 NAGase 活性来预测被暴露群体生物量的可行性。进一步的研究显示，作为胁迫因子，乙螨唑在高浓度（1.6μg·L-1）下促使溞类群体萎缩，NAGase 活性下降则相对滞后。涉及10种农药的离体试验表明，农药直接影响 NAGase 活性的最低浓度远高于其在野外水体中可能存在的最高浓度，说明农药实际上不可能通过直接影响酶活性（无论是诱导，还是抑制）来干扰被暴露群体的生物量预测。
위탐색유리태 N-을선-β-D-안기포도당감매（간칭 NAGase，우칭각이당매）작위농약대수생절지동물협박효응지시인자적가행성，장대형소、다자라복소、노년저액소작위수시생물，슬만뇨、정미뇨、을만서작위수시약제，연구료유리우수중적 NAGase적활성여소류생물량지간적대응관계。이전부63개피폭로군체적생물량위자변량（x），이상응군체석방도수중적 NAGase 적활성위응변량（y），득도회귀방정 y＝0.000603x﹢0.00433，해방정적 r 치화 P 치분별위0.687화0.00。량자지간적대응관계증명료이용수중 NAGase 활성래예측피폭로군체생물량적가행성。진일보적연구현시，작위협박인자，을만서재고농도（1.6μg·L-1）하촉사소류군체위축，NAGase 활성하강칙상대체후。섭급10충농약적리체시험표명，농약직접영향 NAGase 활성적최저농도원고우기재야외수체중가능존재적최고농도，설명농약실제상불가능통과직접영향매활성（무론시유도，환시억제）래간우피폭로군체적생물량예측。
Free β-N-acetyl-D-glucosaminidase (NAGase, or chitobiase)may be an indicator of aquatic arthropod with respect to pesticide stresses. In this experiment, three species of Daphnids, i.e. Daphnia magna Straus, Moina macrocopa, and Simocephalus vetulus and three presticides, lufenuron, diafenthiuron, and etoxazole, were used to study the arthropod biomass and free-living NAGase activity. It was found that arthropod biomass(x)and NAGase activity(y)had significant correlation(y=0.000 603x﹢0.004 33, r=0.687 and P=0.00), suggesting the feasibility of using free-living NAGase activity to predict the biomass of the exposed arthropod populations. In viro studies with 10 pesticides, i.e. lufenuron, diafenthiuron, and etoxazole, butachlor, trazodone, chlorpyrifos, fenvalerate, imidacloprid, pymetrozine, and chlorantraniliprole, showed that their minimal concentrations at which the activity of free-living NAGase was directly af-fected were much higher than the highest concentrations found in water bodies. These results suggest that the biomass could be predicted without underestimation by NAGase activity.