CAJ | 학술논문

为了研究氨氮毒性作用对脊尾白虾（Exopalaemon carinicauda）抗氧化及脂质过氧化作用的影响，将脊尾白虾暴露于低浓度氨氮（4.79±0.12）mg·L-1、中浓度氨氮（9.05±0.18）mg·L-1、中高浓度氨氮（17.64±0.25）mg·L-1和高浓度氨氮（34.87±0.46）mg·L-1中72 h，于胁迫后1、3、6、12、24、48、72 h 测定血淋巴和肝胰腺中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、丙二醛（MDA）含量以及 GPx 基因相对表达量，结果显示：除低浓度氨氮组外，氨氮胁迫6~24 h，脊尾白虾血淋巴和肝胰腺组织 SOD、CAT 活力都有不同程度的升高，胁迫48~72 h 上述指标则均被抑制；氨氮胁迫过程中血淋巴 GPx 活力和 GPx 基因表达显著高于对照组（P﹤0.05），而肝胰腺中 GPx 基因表达在48~72 h 有所下降；血淋巴和肝胰腺中 MDA 含量随着胁迫时间的延长呈现上升的趋势，且上述指标在肝胰腺中的活力较血淋巴高。实验结果表明，氨氮胁迫3~24 h 对脊尾白虾 SOD 和 CAT 活力具有一定的诱导作用，但胁迫48~72 h 则抑制其活性；脂质过氧化产物 MDA 含量在整个胁迫过程中有增加的趋势；肝胰腺可能是脊尾白虾主要的氨氮代谢中心。
위료연구안담독성작용대척미백하（Exopalaemon carinicauda）항양화급지질과양화작용적영향，장척미백하폭로우저농도안담（4.79±0.12）mg·L-1、중농도안담（9.05±0.18）mg·L-1、중고농도안담（17.64±0.25）mg·L-1화고농도안담（34.87±0.46）mg·L-1중72 h，우협박후1、3、6、12、24、48、72 h 측정혈림파화간이선중초양화물기화매（SOD）、과양화경매（CAT）、곡광감태과양화물매（GPx）、병이철（MDA）함량이급 GPx 기인상대표체량，결과현시：제저농도안담조외，안담협박6~24 h，척미백하혈림파화간이선조직 SOD、CAT 활력도유불동정도적승고，협박48~72 h 상술지표칙균피억제；안담협박과정중혈림파 GPx 활력화 GPx 기인표체현저고우대조조（P﹤0.05），이간이선중 GPx 기인표체재48~72 h 유소하강；혈림파화간이선중 MDA 함량수착협박시간적연장정현상승적추세，차상술지표재간이선중적활력교혈림파고。실험결과표명，안담협박3~24 h 대척미백하 SOD 화 CAT 활력구유일정적유도작용，단협박48~72 h 칙억제기활성；지질과양화산물 MDA 함량재정개협박과정중유증가적추세；간이선가능시척미백하주요적안담대사중심。
Ammonia is a common toxic substance in the aquaculture environment. However, there is little information about the effects of ammonia on antioxidant system and lipid peroxidation in ridgetail white prawn Exopalaemon carinicauda. In this study, the prawns were ex-posed to different concentrations of ammonia(4.79±0.12)mg·L-1,(9.05±0.18)mg·L-1,(17.64±0.25)mg·L-1, and(34.87±0.46)mg·L-1 for up to 72 h. Activities of superoxide dismutase(SOD), catalase(CAT), and glutathione peroxidase(GPx), malondialdehyde(MDA)content, and GPx gene expression were assessed in haemocytes and hepatopancreas at 1, 3, 6, 12, 24, 48 and 72 h after ammonia stresses. In all am-monia stresses except low concentration, activities of SOD and CAT in haemocytes and hepatopancreas increased between 6h to 24 h after ammonia stresses, but reduced at 48 h and 72 h, as compared with the control. The GPx activity and GPx gene expression in haemocytes were significantly higher in the treatments than in the control (P﹤0.05), but the expression level of GPx gene in hepatopancreas decreased under ammonia stresses. Contents of MDA in haemocytes and hepatopancreas gradually increased with increasing ammonia stresses, with higher MDA in hepatopancreas than in haemocytes. The results also showed that SOD and CAT in E. carinicauda were induced at 3~24 h,but inhibited at 48~72 h after ammonia stresses and that lipid peroxidative product MDA increased under ammonia stresses. The hep-atopancreas might be a sensitive tissue to ammonia stress.