CAJ | 학술논문

目的：探讨白介素-6（IL-6）及基质金属蛋白酶-2（MMP-2）在胚胎发育中的可能作用。方法：对30例不明原因胚胎停止发育病例（研究组）和30例正常早孕吸宫流产病例（对照组）的绒毛组织及血清进行研究。采用实时荧光定量逆转录聚合酶链式反应技术（Real time-PCR）和免疫组织化学技术（SP 法）检测两组绒毛组织中 IL-6和 MMP-2 mRNA 的表达和蛋白定位、表达强度；采用双抗体酶联免疫夹心法（ELISA法）测定IL-6在两组血清中的浓度。结果：绒毛组织中，IL-6与MMP-2在合体及绒毛滋养层细胞中均有表达，Real time-PCR 及免疫组化结果均显示IL-6在研究组中的表达水平低于对照组，MMP-2在研究组中的表达水平高于对照组，差异均有统计学意义（P＜0．05）； IL-6在血清中的表达水平组间无统计学差异（P＞0．05）。结论：孕早期绒毛组织中IL-6和MMP-2的正常表达对维持早期胚胎发育起到一定作用。
목적：탐토백개소-6（IL-6）급기질금속단백매-2（MMP-2）재배태발육중적가능작용。방법：대30례불명원인배태정지발육병례（연구조）화30례정상조잉흡궁유산병례（대조조）적융모조직급혈청진행연구。채용실시형광정량역전록취합매련식반응기술（Real time-PCR）화면역조직화학기술（SP 법）검측량조융모조직중 IL-6화 MMP-2 mRNA 적표체화단백정위、표체강도；채용쌍항체매련면역협심법（ELISA법）측정IL-6재량조혈청중적농도。결과：융모조직중，IL-6여MMP-2재합체급융모자양층세포중균유표체，Real time-PCR 급면역조화결과균현시IL-6재연구조중적표체수평저우대조조，MMP-2재연구조중적표체수평고우대조조，차이균유통계학의의（P＜0．05）； IL-6재혈청중적표체수평조간무통계학차이（P＞0．05）。결론：잉조기융모조직중IL-6화MMP-2적정상표체대유지조기배태발육기도일정작용。
Objective To explore the relationship between the Interleukin 6 , matrix metalloproteinases 2 and early embryo arrest. Methods Real time-PCR was used to measure the mRNA expression of IL-6 and MMP-2 and immunohistochemistry (IHC, SP method)was used to measure the location and expression of the two different kinds of protein in villus. ELISA was used to measure the level of IL-6 in serum. Results Real-time PCR and IHC showed that the expression levels of IL-6 was significantly lower in experimental group than in control group, and MMP-2 was significantly higher than the control group (P < 0.05). Differenc of IL-6 level in serum between the two groups was not statistically significant (P > 0.05). Conclusion Proper expressions of IL-6 and MMP-2 in the villus tissue play a key role in the maintenance of early pregnancy.