CAJ | 학술논문

目的：探讨caspase-3活化对热打击诱导人脐静脉内皮细胞凋亡的影响。方法：建立人脐静脉内皮细胞热打击模型，对照组将细胞置于标准37℃、5％CO2细胞培养箱，热打击组将细胞置于39℃、41℃、43℃细胞培养箱中进行热打击2 h，热打击后继续在细胞培养箱孵育24 h。使用Hoechst33258荧光染色检测细胞凋亡、Western blot 检测caspase-3蛋白表达、同时检测caspase-3抑制剂Z-DEVD-FMK对热打击组细胞凋亡的影响。结果：与对照组比较，39℃热打击对内皮细胞凋亡无影响，随着热打击温度的增加人脐静脉内皮细胞凋亡明显增多、caspase-3蛋白表达明显增加，caspase-3抑制剂 Z-DEVD-FMK 明显抑制了热打击诱导的人脐静脉内皮细胞凋亡及 caspase-3蛋白的表达。结论： caspase-3介导了热打击诱导的人脐静脉细胞的凋亡。
목적：탐토caspase-3활화대열타격유도인제정맥내피세포조망적영향。방법：건립인제정맥내피세포열타격모형，대조조장세포치우표준37℃、5％CO2세포배양상，열타격조장세포치우39℃、41℃、43℃세포배양상중진행열타격2 h，열타격후계속재세포배양상부육24 h。사용Hoechst33258형광염색검측세포조망、Western blot 검측caspase-3단백표체、동시검측caspase-3억제제Z-DEVD-FMK대열타격조세포조망적영향。결과：여대조조비교，39℃열타격대내피세포조망무영향，수착열타격온도적증가인제정맥내피세포조망명현증다、caspase-3단백표체명현증가，caspase-3억제제 Z-DEVD-FMK 명현억제료열타격유도적인제정맥내피세포조망급 caspase-3단백적표체。결론： caspase-3개도료열타격유도적인제정맥세포적조망。
Objective To explore the effect of activation of caspase-3 on apoptosis of human umbilical vein endothelial cells induced by heat stress. Methods A model of human umbilical vein endothelial cell heat stress was established. The endothelial cells in the control group were incubated at 37 ℃, while the cells in the heat stress group were incubated at 39 ℃, 41 ℃, or 43 ℃ for 2 h , then all the cells were further incubated at 37 ℃ for 24 h. Apoptosis was detected using staining with Hoechst33258; protein levels of caspase-3 were analyzed by Western blot; and the effect of caspase-3 inhibitor Z-DEVD-FMK on heat stress-induced apoptosis was determined. Results As compared with the control group, with an elevation in heat stress temperature (41℃ to 43 ℃), apoptosis was markedly increased and level of caspase-3 was significantly increased. In addition , caspase-3 inhibitor Z-DEVD-FMK significantly decreased the heat stress-induced apoptosis and levels of caspase-3. Conclusions Caspase-3 mediates apoptosis of human umbilical vein endothelial cells can be induced by heat stress.