CAJ | 학술논문

目的：探讨细胞间粘附分子-1（ICAM-1）基因K469E多态性及血小板内皮细胞粘附分子-1（PECAM-1）基因C373G多态性各等位基因及基因型在子痫前期患者及健康孕妇中的分布频率，分析上述基因多态性与子痫前期的关系。方法采用聚合酶链反应-限制性片段多态性（PCR-RFLP）方法和DNA序列测序法对110名子痫前期患者及110名健康足月孕妇ICAM-1基因K469E位点及PECAM-1基因C373G位点进行检测，同时比较两组人群中的相关的临床资料。结果 ICAM-1基因K469E位点及PECAM-1基因C373G位点基因频率分布符合Hardy-Weinberg平衡定律，具有群体代表性。ICAM-1基因K469E位点基因型频率及等位基因频率在两组人群中均无显著差异（P>0.05）。PECAM-1基因C373G位点CC基因型和CG基因型在两组人群分布中存在显著差异（P<0.05）。CG基因型携带者患子痫前期的风险是CC基因型的1.959倍（OR=1.959，95%CI：1.090-3.520， P=0.024），且通过Logistic回归分析发现该相关性独立于孕妇年龄、孕次、产次及孕前BMI。C等位基因频率和G等位基因频率在两组人群的分布差异无统计学意义（P>0.05）。结论 PECAM-1基因C373G位点CG基因型携带者可能与子痫前期患者较高的遗传易感性相关，而ICAM-1基因K469E多态性则与子痫前期的发病无相关。
목적：탐토세포간점부분자-1（ICAM-1）기인K469E다태성급혈소판내피세포점부분자-1（PECAM-1）기인C373G다태성각등위기인급기인형재자간전기환자급건강잉부중적분포빈솔，분석상술기인다태성여자간전기적관계。방법채용취합매련반응-한제성편단다태성（PCR-RFLP）방법화DNA서렬측서법대110명자간전기환자급110명건강족월잉부ICAM-1기인K469E위점급PECAM-1기인C373G위점진행검측，동시비교량조인군중적상관적림상자료。결과 ICAM-1기인K469E위점급PECAM-1기인C373G위점기인빈솔분포부합Hardy-Weinberg평형정률，구유군체대표성。ICAM-1기인K469E위점기인형빈솔급등위기인빈솔재량조인군중균무현저차이（P>0.05）。PECAM-1기인C373G위점CC기인형화CG기인형재량조인군분포중존재현저차이（P<0.05）。CG기인형휴대자환자간전기적풍험시CC기인형적1.959배（OR=1.959，95%CI：1.090-3.520， P=0.024），차통과Logistic회귀분석발현해상관성독립우잉부년령、잉차、산차급잉전BMI。C등위기인빈솔화G등위기인빈솔재량조인군적분포차이무통계학의의（P>0.05）。결론 PECAM-1기인C373G위점CG기인형휴대자가능여자간전기환자교고적유전역감성상관，이ICAM-1기인K469E다태성칙여자간전기적발병무상관。
Objective To investigate the distributions of genotypic and allelic frequencies of intercellular adhesion molecule-1 (ICAM-1) gene K469E and platelet endothelial cell adhesion molecule-1 (PECAM-1) gene C373G in patients with preeclampsia. Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and DNA sequencing were used for detecting ICAM-1 gene K469E and PECAM-1 gene C373G genotypes in 110 women with preeclampsia and 110 normotensive pregnant women in comparison with their clinical characteristics. Results The distributions of observed and expected genotype frequencies were consistent with Hardy-Weinberg equilibrium. No significant differences were found in the genotype and allele frequencies of ICAM-1 gene K469E between the two groups (P>0.05), but the CC and the CG genotype frequencies of PECAM-1 gene C373G were significantly different between them (P<0.05). The relative risk for preeclampsia of CG genotype was 1.959 folds of that in CC genotype carriers (OR=1.959, 95%CI:1.090-3.520, P=0.024), and this association still existed after adjustment for age, gravidity, parity and BMI in logistic regression models. The C373G allele frequencies showed no significant difference between the two groups (P>0.05). Conclusions The CG genotype of PECAM-1 gene C373G genetically predispose the carriers to preeclampsia, while ICAM-1gene K469E polymorphisms is not associated with preeclampsia.