CAJ | 학술논문

目的探讨内质网氧化还原酶1-Lα（ Ero1-Lα）在高血压导致内皮细胞损伤中的变化，从而进一步阐明内质网应激在高血压发生发展过程中的重要作用。方法利用不同浓度血管紧张素Ⅱ（ AngⅡ）持续刺激培养的人脐静脉内皮细胞（ HUVECs）24 h模拟高血压状态。利用MTT法检测细胞的凋亡率。利用免疫荧光化学和Western blot法检测Ero1-Lα在内皮细胞中的表达。结果 AngⅡ作用24 h后的HUVECs出现凋亡。随着AngⅡ的浓度升高，HUVECs的凋亡增加，Ero1-Lα的Western blot表达和免疫荧光平均光密度值明显上升。结论 AngⅡ可诱导HUVECs凋亡，Ero1-Lα的高表达参与了AngⅡ诱导的HUVECs凋亡。
목적탐토내질망양화환원매1-Lα（ Ero1-Lα）재고혈압도치내피세포손상중적변화，종이진일보천명내질망응격재고혈압발생발전과정중적중요작용。방법이용불동농도혈관긴장소Ⅱ（ AngⅡ）지속자격배양적인제정맥내피세포（ HUVECs）24 h모의고혈압상태。이용MTT법검측세포적조망솔。이용면역형광화학화Western blot법검측Ero1-Lα재내피세포중적표체。결과 AngⅡ작용24 h후적HUVECs출현조망。수착AngⅡ적농도승고，HUVECs적조망증가，Ero1-Lα적Western blot표체화면역형광평균광밀도치명현상승。결론 AngⅡ가유도HUVECs조망，Ero1-Lα적고표체삼여료AngⅡ유도적HUVECs조망。
Objective To investigate the role of endoplasmic oxidoreductin -1-like protein alpha ( Ero1-Lα) in hypertension-induced vein endothelium injury , and further to confirm the vital role of endoplasmic reticulum ( ER) stress in the development of hypertension .Methods Human umbilical vein endothelial cells ( HUVECs) were cultured in hypertension mimic status via culturing with different concentrations of angiotensin Ⅱ( AngⅡ) .MTT was used to investi-gate the apoptosis rate of HUVECs , while Western blot and immunofluorescence histochemistry were applied to assess the expression of Ero1-Lα.Results The apoptosis of HUVECs was observed 24 h after stimulus of Ang Ⅱ.With the AngⅡconcentration rising , increase in the apoptosis rate of HUVECs , and Western blot expression and average immunofluo-rescence optical density of Ero 1-Lαwere revealed .Conclusion Ang Ⅱ can induce apoptosis of HUVECs , in which high expression of Ero 1-Lαparticipates .