广东医学
廣東醫學
엄동의학
GUNAGDONG MEDICAL JOURNAL
2014年
6期
812-815
,共4页
宗少晖%曾高峰%方晔%邹斌%高太行
宗少暉%曾高峰%方曄%鄒斌%高太行
종소휘%증고봉%방엽%추빈%고태행
脊髓损伤%STAT3%白细胞介素-17%白细胞介素-6%白细胞介素-21
脊髓損傷%STAT3%白細胞介素-17%白細胞介素-6%白細胞介素-21
척수손상%STAT3%백세포개소-17%백세포개소-6%백세포개소-21
spinal cord injury%signal transducer and activator of transcription 3%interleukin-17%interleukin-6%interleukin-21
目的：观察大鼠脊髓损伤模型中磷酸化信号转导与转录激活因子3（ p-STAT3）及Th17相关因子的水平，初步探讨STAT3对脊髓损伤后Th17细胞的作用。方法成年健康雄性SD大鼠75只随机分为对照组（n＝15）和模型组（每个时点n＝15）。模型组采用改良Allen氏打击法建立脊髓损伤模型，对照组仅行椎板全切除术。在建模后1、24、48和72 h获取模型组大鼠损伤段脊髓、脾脏及外周血，免疫组织化学染色法检测白细胞介素（ IL）-17在损伤段脊髓组织中的分布及表达情况，蛋白免疫印迹法检测p-STAT3在损伤段脊髓和脾脏组织中的表达变化，ELISA法检测外周血IL-6、IL-21和IL-23的动态变化，并与对照组大鼠作比较。结果模型组各时点IL-17、p-STAT3、IL-6、IL-21和IL-23的表达明显高于对照组（P＜0.05），相关性分析显示p-STAT3的表达均与IL-17、IL-6及IL-21的表达呈正相关性（ P＜0.05）。结论 STAT3的异常激活可能是造成脊髓损伤后Th17细胞过度表达的重要原因。
目的：觀察大鼠脊髓損傷模型中燐痠化信號轉導與轉錄激活因子3（ p-STAT3）及Th17相關因子的水平，初步探討STAT3對脊髓損傷後Th17細胞的作用。方法成年健康雄性SD大鼠75隻隨機分為對照組（n＝15）和模型組（每箇時點n＝15）。模型組採用改良Allen氏打擊法建立脊髓損傷模型，對照組僅行椎闆全切除術。在建模後1、24、48和72 h穫取模型組大鼠損傷段脊髓、脾髒及外週血，免疫組織化學染色法檢測白細胞介素（ IL）-17在損傷段脊髓組織中的分佈及錶達情況，蛋白免疫印跡法檢測p-STAT3在損傷段脊髓和脾髒組織中的錶達變化，ELISA法檢測外週血IL-6、IL-21和IL-23的動態變化，併與對照組大鼠作比較。結果模型組各時點IL-17、p-STAT3、IL-6、IL-21和IL-23的錶達明顯高于對照組（P＜0.05），相關性分析顯示p-STAT3的錶達均與IL-17、IL-6及IL-21的錶達呈正相關性（ P＜0.05）。結論 STAT3的異常激活可能是造成脊髓損傷後Th17細胞過度錶達的重要原因。
목적：관찰대서척수손상모형중린산화신호전도여전록격활인자3（ p-STAT3）급Th17상관인자적수평，초보탐토STAT3대척수손상후Th17세포적작용。방법성년건강웅성SD대서75지수궤분위대조조（n＝15）화모형조（매개시점n＝15）。모형조채용개량Allen씨타격법건립척수손상모형，대조조부행추판전절제술。재건모후1、24、48화72 h획취모형조대서손상단척수、비장급외주혈，면역조직화학염색법검측백세포개소（ IL）-17재손상단척수조직중적분포급표체정황，단백면역인적법검측p-STAT3재손상단척수화비장조직중적표체변화，ELISA법검측외주혈IL-6、IL-21화IL-23적동태변화，병여대조조대서작비교。결과모형조각시점IL-17、p-STAT3、IL-6、IL-21화IL-23적표체명현고우대조조（P＜0.05），상관성분석현시p-STAT3적표체균여IL-17、IL-6급IL-21적표체정정상관성（ P＜0.05）。결론 STAT3적이상격활가능시조성척수손상후Th17세포과도표체적중요원인。
Objective To investigate the expression of p -STAT3(signal transducer and activator of transcription 3) and Th17 cell related signal molecules in the rat model of spinal cord injury , so as to explore the roles of STAT3 and its related signal molecules in spinal cord injury .Methods A total of 75 adult male SD rats were randomly divided into two groups, control group (n=15) and model group (n=15 each time point).Acute spinal cord injury rat model was estab-lished by the modified Allen weight drop method in the model group , while laminectomy alone was performed in control group.Each 15 rats in model group were sacrificed at the 1st, 24th, 48th and 72nd hour after operation for collection of spi-nal cord tissues, spleen tissues, and peripheral blood.Immunohistochemistry method was used to detect the expressions of interleukin ( IL)-17 in damaged spinal cord tissues , Western blot was applied to assess the protein expression of p -STAT3 in the spinal cord and spleen tissues , and ELISA was used to measure serum IL -6, IL-21 and IL-23.Results Expression of IL-17, p-STAT3, IL-6, IL-21 and IL-23 in model group was significantly higher than control group (P<0.05).Furthermore, the expression of p -STAT3 was significantly positively correlated with IL -17, IL-6 and IL-21 (P<0.05).Conclusion Aberrant activation of STAT3 may be one of factors to contribute to Th17 polariza-tion after spinal cord injury .
