广东医学
廣東醫學
엄동의학
GUNAGDONG MEDICAL JOURNAL
2014年
6期
805-808
,共4页
孙传伟%陈华德%卞徽宁%郑少逸%刘族安%赖文
孫傳偉%陳華德%卞徽寧%鄭少逸%劉族安%賴文
손전위%진화덕%변휘저%정소일%류족안%뢰문
脂肪来源间充质干细胞%全层皮肤缺损%创面愈合
脂肪來源間充質榦細胞%全層皮膚缺損%創麵愈閤
지방래원간충질간세포%전층피부결손%창면유합
adipose-derived stem cells%full thickness cutaneous deficiency%wound healing
目的:探讨脂肪来源间充质干细胞( ADSCs)移植在全层皮肤缺损修复中的作用。方法25只SD大鼠制作大鼠背部全层皮肤缺损模型,随机分为3组,分别移植胶原膜(胶原膜组)、ADSCs+胶原膜( ADSCs+胶原膜组)后覆盖异体SD大鼠皮肤及直接以异体SD大鼠皮肤覆盖(空白对照组)。观察各组创口的愈合速度,并通过愈合皮肤抗拉力实验、HE染色、原位杂交的方法检测愈合后皮肤的抗拉性、组织学改变及ADSCs的表达。结果 ADSCs及胶原膜对大鼠一般情况无明显影响;ADSCs+胶原膜组创面愈合率为(94.24±3.13)%,显著高于空白对照组的(84.57±2.42)%及胶原膜组的(84.72±9.70)%,差异有统计学意义(P<0.05);ADSCs+胶原膜组皮肤的最大拉力、弹性位移和拉伸率与空白对照组及胶原膜组比较差异无统计学意义( P>0.05);ADSCs+胶原膜组皮肤真皮层中存在大量类似于正常皮肤的毛囊结构;在ADSCs+胶原膜组愈合创面皮肤组织中可追踪到雄性大鼠来源细胞的阳性表达。结论 ADSCs能促进大鼠全层皮肤缺损的修复。
目的:探討脂肪來源間充質榦細胞( ADSCs)移植在全層皮膚缺損脩複中的作用。方法25隻SD大鼠製作大鼠揹部全層皮膚缺損模型,隨機分為3組,分彆移植膠原膜(膠原膜組)、ADSCs+膠原膜( ADSCs+膠原膜組)後覆蓋異體SD大鼠皮膚及直接以異體SD大鼠皮膚覆蓋(空白對照組)。觀察各組創口的愈閤速度,併通過愈閤皮膚抗拉力實驗、HE染色、原位雜交的方法檢測愈閤後皮膚的抗拉性、組織學改變及ADSCs的錶達。結果 ADSCs及膠原膜對大鼠一般情況無明顯影響;ADSCs+膠原膜組創麵愈閤率為(94.24±3.13)%,顯著高于空白對照組的(84.57±2.42)%及膠原膜組的(84.72±9.70)%,差異有統計學意義(P<0.05);ADSCs+膠原膜組皮膚的最大拉力、彈性位移和拉伸率與空白對照組及膠原膜組比較差異無統計學意義( P>0.05);ADSCs+膠原膜組皮膚真皮層中存在大量類似于正常皮膚的毛囊結構;在ADSCs+膠原膜組愈閤創麵皮膚組織中可追蹤到雄性大鼠來源細胞的暘性錶達。結論 ADSCs能促進大鼠全層皮膚缺損的脩複。
목적:탐토지방래원간충질간세포( ADSCs)이식재전층피부결손수복중적작용。방법25지SD대서제작대서배부전층피부결손모형,수궤분위3조,분별이식효원막(효원막조)、ADSCs+효원막( ADSCs+효원막조)후복개이체SD대서피부급직접이이체SD대서피부복개(공백대조조)。관찰각조창구적유합속도,병통과유합피부항랍력실험、HE염색、원위잡교적방법검측유합후피부적항랍성、조직학개변급ADSCs적표체。결과 ADSCs급효원막대대서일반정황무명현영향;ADSCs+효원막조창면유합솔위(94.24±3.13)%,현저고우공백대조조적(84.57±2.42)%급효원막조적(84.72±9.70)%,차이유통계학의의(P<0.05);ADSCs+효원막조피부적최대랍력、탄성위이화랍신솔여공백대조조급효원막조비교차이무통계학의의( P>0.05);ADSCs+효원막조피부진피층중존재대량유사우정상피부적모낭결구;재ADSCs+효원막조유합창면피부조직중가추종도웅성대서래원세포적양성표체。결론 ADSCs능촉진대서전층피부결손적수복。
Objective To investigate the effects of adipose -derived stem cells ( ADSCs ) transplantation in full thickness cutaneous deficiency repairing in rats .Methods Full thickness cuntaneous deficiency models were constructed in 25 SD rats and randomly divided into control group , collagen membrane group ( negative control ) and ADSCs-collagen membrane group;in which allogeneic skin graft ( ASG) alone, ASG plus collagen membrane , and ASG plus ADSCs-col-lagen membrane were given for wound covering , respectively.The level of wound healing was observed .Tensile test, HE staining and in situ hybridization were carried out to assess the tensile strength , histological changes and ADSCs′expres-sion in new skin , respectively .Results Animal experiment showed that ADSCs and collagen membrane transplantations were safe.The wound healing rate of ADSCs -collagen membrane group [(94.24 ±3.13)%] was significantly higher than those of control group [(84.57 ±2.42)%] and collagen membrane group [(84.72 ±9.70)%].There was no sig-nificant difference was revealed in the tensile strength of new skin among the three groups ( P >0.05 ) .HE staining showed that there were neogenetic skin appendages in experimental group rather than controls .According to in situ hybrid-ization, SRY DNA was positive in the new skin of ADSCs -collagen membrane group .Conclusion ADSCs transplanta-tion can promote the full thickness cutaneous deficiency repairing in rats .