中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
5期
906-909
,共4页
白细胞介素4%树突细胞%抗原,CD11c%抗原,CD80%抗原,CD86
白細胞介素4%樹突細胞%抗原,CD11c%抗原,CD80%抗原,CD86
백세포개소4%수돌세포%항원,CD11c%항원,CD80%항원,CD86
Interleukin-4%Dendritic cells%Antigens,CD11c%Antigens,CD80%Antigens,CD86
目的：探讨IL-4对小鼠骨髓树突状细胞（DC细胞）表面分子CD11c、CD80、CD86表达的影响及其意义。方法对照组（n＝5只，30孔）应用20 ng/ml GM-CSF，实验组（n＝5只，30孔）应用20 ng/ml GM-CSF＋20 ng/ml IL-4分别刺激小鼠骨髓细胞生长，隔日进行细胞换液，观察并对比细胞形态学变化，流式细胞仪测定 DC 细胞表面相关分子表达。结果实验组诱导小鼠骨髓细胞第7日观察可见DC细胞形态，对照组第7日未发现明显DC细胞形态，流式细胞仪测定实验组 CD11c（0.546±0.289）、CD80（0.506±0.085）、CD86（0.562±0.260）表达分别较对照组CD11c（0.236±0.058）、CD80（0.279±0.096）、CD86（0.237±0.070）表达明显增高（P＜0.05）。结论 IL-4可以促进小鼠骨髓DC细胞表面分子CD11c、CD80、CD86表达，促进DC细胞分化成熟。
目的：探討IL-4對小鼠骨髓樹突狀細胞（DC細胞）錶麵分子CD11c、CD80、CD86錶達的影響及其意義。方法對照組（n＝5隻，30孔）應用20 ng/ml GM-CSF，實驗組（n＝5隻，30孔）應用20 ng/ml GM-CSF＋20 ng/ml IL-4分彆刺激小鼠骨髓細胞生長，隔日進行細胞換液，觀察併對比細胞形態學變化，流式細胞儀測定 DC 細胞錶麵相關分子錶達。結果實驗組誘導小鼠骨髓細胞第7日觀察可見DC細胞形態，對照組第7日未髮現明顯DC細胞形態，流式細胞儀測定實驗組 CD11c（0.546±0.289）、CD80（0.506±0.085）、CD86（0.562±0.260）錶達分彆較對照組CD11c（0.236±0.058）、CD80（0.279±0.096）、CD86（0.237±0.070）錶達明顯增高（P＜0.05）。結論 IL-4可以促進小鼠骨髓DC細胞錶麵分子CD11c、CD80、CD86錶達，促進DC細胞分化成熟。
목적：탐토IL-4대소서골수수돌상세포（DC세포）표면분자CD11c、CD80、CD86표체적영향급기의의。방법대조조（n＝5지，30공）응용20 ng/ml GM-CSF，실험조（n＝5지，30공）응용20 ng/ml GM-CSF＋20 ng/ml IL-4분별자격소서골수세포생장，격일진행세포환액，관찰병대비세포형태학변화，류식세포의측정 DC 세포표면상관분자표체。결과실험조유도소서골수세포제7일관찰가견DC세포형태，대조조제7일미발현명현DC세포형태，류식세포의측정실험조 CD11c（0.546±0.289）、CD80（0.506±0.085）、CD86（0.562±0.260）표체분별교대조조CD11c（0.236±0.058）、CD80（0.279±0.096）、CD86（0.237±0.070）표체명현증고（P＜0.05）。결론 IL-4가이촉진소서골수DC세포표면분자CD11c、CD80、CD86표체，촉진DC세포분화성숙。
Objective To explore the efficacy of IL-4 on the expression of phenotype of CD11c, CD80, CD86 and its underlying meaning in cultured dendritic cells (DC). Methods On the base of the routine culture, both the control group (n=5, 30 holes) and the test group (n=5, 30 holes) of mouse bone marrow cells were added with 20 ng/ml GM-CSF. In addition the test group was further treated with 20 ng/ml of IL-4. After 7 days, both groups were observed under microscope and CD11c, CD80, CD86 were measured with flow cytometry. Results At the 7 days, the DC cells were found under microcopy in the test group but not in the control group. Flow cytometry demonstrated the cell phenotype of CD11c (0.546 ±0.289), CD80(0.506±0.085) and CD86(0.562±0.260) expression in test group were higher than that of the control group, which were CD11c (0.236±0.058), CD80 (0.279±0.096) and CD86 (0.237±0.070), respectively (P<0.05). Conclusions IL-4 could effectively promote the phenotype molecule expression of CD11c,CD80,CD86, and thus stimulate the differentiation and maturation of bone marrow cells to DC cells.
