中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
5期
825-829
,共5页
张娟%张秀莲%张伟华%樊润梅%葛晓静
張娟%張秀蓮%張偉華%樊潤梅%葛曉靜
장연%장수련%장위화%번윤매%갈효정
受体,肿瘤坏死因子%贫血,再生障碍性%细胞凋亡%骨髓单个核细胞
受體,腫瘤壞死因子%貧血,再生障礙性%細胞凋亡%骨髓單箇覈細胞
수체,종류배사인자%빈혈,재생장애성%세포조망%골수단개핵세포
Receptors,tumor necrosis factor%Anemia,aplastic%Apoptosis%Bone marrow mononuclear cell
目的：通过检测肿瘤坏死因子相关凋亡诱导配体（TRAIL）诱导正常骨髓单个核细胞（BMMNC）凋亡率，及抑制TRAIL凋亡途径后再生障碍性贫血（AA）患者骨髓单个核细胞凋亡率，探讨TRAIL在AA患者骨髓造血细胞凋亡中的作用。方法采用流式细胞术检测43例骨髓象正常者（正常组）及TRAIL作用后（TRAIL组）其骨髓单个核细胞凋亡率；14例AA患者（AA组）及TRAIL抗体作用后（TRAIL-Ab组）其骨髓单个核细胞凋亡率。结果（1）AA组凋亡率较正常组明显增高，差异有统计学意义（P＜0.05）。（2）TRAIL组（浓度为30 ng/ml，作用24 h）凋亡率[（39.98±4.594）%]高于正常组[（19.90±6.656）%]，差异有统计学意义（P＜0.05）。（3）TRAIL-Ab组凋亡率[（30.28±4.594）%]较AA组[（51.29±7.355）%]减少，差异有统计学意义（P＜0.05）。结论一定浓度的TRAIL可使正常骨髓单个核细胞凋亡增加，抑制TRAIL诱导的凋亡途径后，AA患者骨髓单个核细胞凋亡减少，提示TRAIL诱导的细胞凋亡，可能在AA发病中发挥一定作用。
目的：通過檢測腫瘤壞死因子相關凋亡誘導配體（TRAIL）誘導正常骨髓單箇覈細胞（BMMNC）凋亡率，及抑製TRAIL凋亡途徑後再生障礙性貧血（AA）患者骨髓單箇覈細胞凋亡率，探討TRAIL在AA患者骨髓造血細胞凋亡中的作用。方法採用流式細胞術檢測43例骨髓象正常者（正常組）及TRAIL作用後（TRAIL組）其骨髓單箇覈細胞凋亡率；14例AA患者（AA組）及TRAIL抗體作用後（TRAIL-Ab組）其骨髓單箇覈細胞凋亡率。結果（1）AA組凋亡率較正常組明顯增高，差異有統計學意義（P＜0.05）。（2）TRAIL組（濃度為30 ng/ml，作用24 h）凋亡率[（39.98±4.594）%]高于正常組[（19.90±6.656）%]，差異有統計學意義（P＜0.05）。（3）TRAIL-Ab組凋亡率[（30.28±4.594）%]較AA組[（51.29±7.355）%]減少，差異有統計學意義（P＜0.05）。結論一定濃度的TRAIL可使正常骨髓單箇覈細胞凋亡增加，抑製TRAIL誘導的凋亡途徑後，AA患者骨髓單箇覈細胞凋亡減少，提示TRAIL誘導的細胞凋亡，可能在AA髮病中髮揮一定作用。
목적：통과검측종류배사인자상관조망유도배체（TRAIL）유도정상골수단개핵세포（BMMNC）조망솔，급억제TRAIL조망도경후재생장애성빈혈（AA）환자골수단개핵세포조망솔，탐토TRAIL재AA환자골수조혈세포조망중적작용。방법채용류식세포술검측43례골수상정상자（정상조）급TRAIL작용후（TRAIL조）기골수단개핵세포조망솔；14례AA환자（AA조）급TRAIL항체작용후（TRAIL-Ab조）기골수단개핵세포조망솔。결과（1）AA조조망솔교정상조명현증고，차이유통계학의의（P＜0.05）。（2）TRAIL조（농도위30 ng/ml，작용24 h）조망솔[（39.98±4.594）%]고우정상조[（19.90±6.656）%]，차이유통계학의의（P＜0.05）。（3）TRAIL-Ab조조망솔[（30.28±4.594）%]교AA조[（51.29±7.355）%]감소，차이유통계학의의（P＜0.05）。결론일정농도적TRAIL가사정상골수단개핵세포조망증가，억제TRAIL유도적조망도경후，AA환자골수단개핵세포조망감소，제시TRAIL유도적세포조망，가능재AA발병중발휘일정작용。
Objective To explore the role of tumor necrosis factor related apoptosis inducing ligand (TRAIL) on aplastic anemia bone marrow mononuclear cell(BMMNC), and approach its effect on the apoptosis of haemopoietic stem cell. By detecting TRAIL induction of normal BMMNC apoptosis rate, and the aplastic anemia BMMNC apoptosis rate after blocking the TRAIL apoptotic pathway. Methods We use flow cytometry to detect the BMMNC apoptosis rate including 43 cases (normal group) and after TRAIL effecting (TRAIL group); 14 cases with aplastic anemia (AA group) and after TRAIL antibody effecting (TRAIL-Ab group). Results (1)Aplastic anemia apoptosis rate was higher than normal group. (2)The apoptosis rate (39.98±4.594)%of TRAIL group(concentration of 30 ng/ml, 24 h) was higher than the normal group (19.90±6.656)%(P<0.05). (3)The apoptosis rate of TRAIL-Ab group (30.28±4.594)%was lower than aplastic anemia group (51.29±7.355)%(P<0.05). Conclusions The TRAIL of a certain concentration can make normal BMMNC apoptosis increases, if block the apoptotic pathway induced by TRAIL, the BMMNC apoptosis of aplastic anemia patients will decrease, suggesting that the cell apoptosis induced by TRAIL may play a role in the onset of aplastic anemia.
