现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2014年
7期
961-962,965
,共3页
黄衍强%莫小强%赵丽娟%陈秉朴%彭素芬%孙芹%胡海萍%杜艺冰%韦荣显
黃衍彊%莫小彊%趙麗娟%陳秉樸%彭素芬%孫芹%鬍海萍%杜藝冰%韋榮顯
황연강%막소강%조려연%진병박%팽소분%손근%호해평%두예빙%위영현
肝肿瘤%肿瘤,实验性%接种%免疫抑制剂%肿瘤移植%小鼠
肝腫瘤%腫瘤,實驗性%接種%免疫抑製劑%腫瘤移植%小鼠
간종류%종류,실험성%접충%면역억제제%종류이식%소서
Liver neoplasm%Neoplasms,experimental%Vaccination%Immunosuppressive agents%Neoplasm transplantation%Mice
目的：探讨构建肝癌移植瘤动物模型的最佳方法。方法选用BALB/c小鼠160只，随机分为地塞米松组、肝癌细胞注射组、地塞米松加肝癌细胞注射组及空白对照组，每组40只，雌、雄各半。地塞米松组在小鼠腹腔内注射地塞米松；肝癌细胞注射组于不同时间在肝脏直接接种H22肝癌细胞株3次；地塞米松加肝癌细胞注射组将上述2种方法联合使用；空白对照组注射生理盐水。1个月后，颈椎脱臼处死小鼠，取其肝组织，计算肝指数，苏木精-伊红染色（HE）后观察肝组织病理变化。结果地塞米松组、空白对照组未发现肝组织癌变，肝癌细胞注射组、地塞米松加肝癌细胞注射组癌变的百分比分别为71.4%（20/28）、91.2%（21/23）。结论肝癌细胞直接接种小鼠肝脏及联合地塞米松干扰均可建立肝癌移植瘤动物模型，但联合地塞米松干扰建模的成功率明显升高。
目的：探討構建肝癌移植瘤動物模型的最佳方法。方法選用BALB/c小鼠160隻，隨機分為地塞米鬆組、肝癌細胞註射組、地塞米鬆加肝癌細胞註射組及空白對照組，每組40隻，雌、雄各半。地塞米鬆組在小鼠腹腔內註射地塞米鬆；肝癌細胞註射組于不同時間在肝髒直接接種H22肝癌細胞株3次；地塞米鬆加肝癌細胞註射組將上述2種方法聯閤使用；空白對照組註射生理鹽水。1箇月後，頸椎脫臼處死小鼠，取其肝組織，計算肝指數，囌木精-伊紅染色（HE）後觀察肝組織病理變化。結果地塞米鬆組、空白對照組未髮現肝組織癌變，肝癌細胞註射組、地塞米鬆加肝癌細胞註射組癌變的百分比分彆為71.4%（20/28）、91.2%（21/23）。結論肝癌細胞直接接種小鼠肝髒及聯閤地塞米鬆榦擾均可建立肝癌移植瘤動物模型，但聯閤地塞米鬆榦擾建模的成功率明顯升高。
목적：탐토구건간암이식류동물모형적최가방법。방법선용BALB/c소서160지，수궤분위지새미송조、간암세포주사조、지새미송가간암세포주사조급공백대조조，매조40지，자、웅각반。지새미송조재소서복강내주사지새미송；간암세포주사조우불동시간재간장직접접충H22간암세포주3차；지새미송가간암세포주사조장상술2충방법연합사용；공백대조조주사생리염수。1개월후，경추탈구처사소서，취기간조직，계산간지수，소목정-이홍염색（HE）후관찰간조직병리변화。결과지새미송조、공백대조조미발현간조직암변，간암세포주사조、지새미송가간암세포주사조암변적백분비분별위71.4%（20/28）、91.2%（21/23）。결론간암세포직접접충소서간장급연합지새미송간우균가건립간암이식류동물모형，단연합지새미송간우건모적성공솔명현승고。
Objective To explore the optimum method of establishing hepatocarcinoma-bearing mice′s model of trans-plantation tumor. Methods Totally 160 BALB/c mice were selected and randomized into dexamethasone group ,hepatoma carci-noma cell injection group,dexamethasone and hepatoma carcinoma cell injection group and blank control group,40 cases in each group and sex in half. The dexamethasone group was with intraperitoneal injection of dexamethasone;the hepatoma carcinoma cell injection group was directly inoculated hepatoma carcinoma cell line H22 in liver three times at different times;the dexamethasone and hepatoma carcinoma cell injection group combined with the above two methods and the blank control group was injected with normal saline. One month later,the mice were killed by cervical dislocation to extract liver tissue,calculate the liver index and ob-serve the pathological changes of liver tissue after hematoxylin-eosin(HE) staining. Results The cancer was not found in dex-amethasone group and control blank group. The percentage of carcinogenesis in liver tissue injection group ,dexamethasone and hepatoma carcinoma cell injection group was 71.4%(20/28) and 91.2%(21/23) respectively. Conclusion Hepatocarcinoma-bearing mice′s model of transplantation tumor can be established by inoculating mice hepatoma carcinoma cell directly and interfer-ence of dexamethasone combined with cyclophosphamide ,moreover combination with dexamethasone interference can improve the success rate of modeling significantly.
