CAJ | 학술논문

目的：探讨肝癌组织中乏氧诱导因子-1α(HIF-1α)蛋白的表达与多药耐药蛋白(multi-drug resistance protein,MRP)的关系。方法收集78例原发性肝细胞癌患者肝组织和对应癌旁正常肝组织的石蜡标本,采用免疫组织化学 SP法检测原发性肝细胞癌组织及其癌旁正常肝组织中 HIF-1α和 MRP 蛋白的表达情况。结果 HIF-1α和MRP蛋白在肝癌组织中的阳性表达率明显高于癌旁正常肝组织(P<0.05)。病理分级(Ⅲ~Ⅳ级)、TNM 分期(Ⅲ~Ⅳ期)、有门静脉癌栓、有淋巴结转移的肝癌组织中 HIF-1α的阳性表达率明显增高(P<0.05)；病理分级(Ⅲ~Ⅳ级)、有淋巴结转移的肝癌组织中 MRP的阳性表达率明显增高(P<0.05)。78份肝癌组织中,HIF-1α和 MRP 的表达之间呈正相关(r=0.524,P<0.05)。结论 HIF-1α和 MRP过表达可能在肝癌的恶性增殖、分化及淋巴结转移过程中发挥重要作用,且两者在肝癌组织中的表达可能具有协同作用。
목적：탐토간암조직중핍양유도인자-1α(HIF-1α)단백적표체여다약내약단백(multi-drug resistance protein,MRP)적관계。방법수집78례원발성간세포암환자간조직화대응암방정상간조직적석사표본,채용면역조직화학 SP법검측원발성간세포암조직급기암방정상간조직중 HIF-1α화 MRP 단백적표체정황。결과 HIF-1α화MRP단백재간암조직중적양성표체솔명현고우암방정상간조직(P<0.05)。병리분급(Ⅲ~Ⅳ급)、TNM 분기(Ⅲ~Ⅳ기)、유문정맥암전、유림파결전이적간암조직중 HIF-1α적양성표체솔명현증고(P<0.05)；병리분급(Ⅲ~Ⅳ급)、유림파결전이적간암조직중 MRP적양성표체솔명현증고(P<0.05)。78빈간암조직중,HIF-1α화 MRP 적표체지간정정상관(r=0.524,P<0.05)。결론 HIF-1α화 MRP과표체가능재간암적악성증식、분화급림파결전이과정중발휘중요작용,차량자재간암조직중적표체가능구유협동작용。
Objective To explore the correlation between the expression of HIF-1 α and multi-drug resistance protein (MRP)in hepatocellular carcinoma (HCC)tissues.Methods The paraffin specimens of 78 HCC cases and the corresponding para-tumor normal liver tissues were collected,and immunohistochemistry SP method was used to detect the expression of HIF-1α and MRP protein in HCC tissues and para-tumor liver tissues.Results The positive rate of HIF-1αand MRP protein in HCC tissues was obviously higher that in para-tumor normal liver tissues(P<0.05).In HCC tissues of pathological grading Ⅲ-Ⅳ and TNM staging Ⅲ-Ⅳ with portal vein tumor thrombus and lymphatic metastasis,the positive rate of HIF-1α clearly increased(P<0.05).The positive rate of MRP showed obvious increase in HCC tissues of pathological grading Ⅲ-Ⅳwith lymphatic metastasis(P<0.05).The expression of HIF-1αand MRP protein in 78 cases of HCC tissues was positively correlated (r=0.524,P<0.05).Conclusion Overexpression of HIF-1αand MRP protein might play a key role in the malignant proliferation,differentiation and lymphatic metastasis of HCC,and synergy may exist in the expression of the two proteins in HCC.