CAJ | 학술논문

目的探讨基质金属蛋白酶-8( MMP-8)在急性Stan-ford A型主动脉夹层中的表达及临床意义。方法30例急性Stanford A型主动脉夹层患者为实验组,30例同期接受单纯主动脉瓣置换的患者为对照组,采用 ELISA 法、Western blot法、免疫组化法等检测MMP-8在实验组和对照组血浆及升主动脉壁中的表达。结果主动脉夹层患者血浆中MMP-8的表达[(106.51±28.27) ng/ml ]明显高于对照组[(25.65±12.95)ng/ml],差异有统计学意义(P<0.05)；实验组升主动脉中层病变显著,炎性细胞浸润夹层边缘。弹性纤维与胶原纤维层变性、排列紊乱、破碎、崩解。其主动脉壁中层MMP-8大量表达。而对照组主动脉壁结构完整,弹性目的探讨基质金属蛋白酶-8( MMP-8)在急性Stan-ford A型主动脉夹层中的表达及临床意义。方法30例急性Stanford A型主动脉夹层患者为实验组,30例同期接受单纯主动脉瓣置换的患者为对照组,采用 ELISA 法、Western blot法、免疫组化法等检测MMP-8在实验组和对照组血浆及升主动脉壁中的表达。结果主动脉夹层患者血浆中MMP-8的表达[(106.51±28.27) ng/ml ]明显高于对照组[(25.65±12.95)ng/ml],差异有统计学意义(P<0.05)；实验组升主动脉中层病变显著,炎性细胞浸润夹层边缘。弹性纤维与胶原纤维层变性、排列紊乱、破碎、崩解。其主动脉壁中层MMP-8大量表达。而对照组主动脉壁结构完整,弹性纤维与胶原纤维排列紧密有序,无中层退行性变。 MMP-8在其主动脉中层仅少量表达或不表达。结论 MMP-8在病变的升主动脉中层内高表达,在正常的弹性纤维层低表达,提示其在主动脉疾病的发生发展中可能发挥作用。
목적탐토기질금속단백매-8( MMP-8)재급성Stan-ford A형주동맥협층중적표체급림상의의。방법30례급성Stanford A형주동맥협층환자위실험조,30례동기접수단순주동맥판치환적환자위대조조,채용 ELISA 법、Western blot법、면역조화법등검측MMP-8재실험조화대조조혈장급승주동맥벽중적표체。결과주동맥협층환자혈장중MMP-8적표체[(106.51±28.27) ng/ml ]명현고우대조조[(25.65±12.95)ng/ml],차이유통계학의의(P<0.05)；실험조승주동맥중층병변현저,염성세포침윤협층변연。탄성섬유여효원섬유층변성、배렬문란、파쇄、붕해。기주동맥벽중층MMP-8대량표체。이대조조주동맥벽결구완정,탄성목적탐토기질금속단백매-8( MMP-8)재급성Stan-ford A형주동맥협층중적표체급림상의의。방법30례급성Stanford A형주동맥협층환자위실험조,30례동기접수단순주동맥판치환적환자위대조조,채용 ELISA 법、Western blot법、면역조화법등검측MMP-8재실험조화대조조혈장급승주동맥벽중적표체。결과주동맥협층환자혈장중MMP-8적표체[(106.51±28.27) ng/ml ]명현고우대조조[(25.65±12.95)ng/ml],차이유통계학의의(P<0.05)；실험조승주동맥중층병변현저,염성세포침윤협층변연。탄성섬유여효원섬유층변성、배렬문란、파쇄、붕해。기주동맥벽중층MMP-8대량표체。이대조조주동맥벽결구완정,탄성섬유여효원섬유배렬긴밀유서,무중층퇴행성변。 MMP-8재기주동맥중층부소량표체혹불표체。결론 MMP-8재병변적승주동맥중층내고표체,재정상적탄성섬유층저표체,제시기재주동맥질병적발생발전중가능발휘작용。
Objective To investigate the expression and clinical significance of matrix metalloproteinase-8 ( MMP-8 ) in the acute Stanford A aortic dissection. Methods Blood samples were collected from 30 acute Stanford A aor-tic dissection patients and 30 aortic valve replacement patients. ELISA was used to measure the contents of MMP-8 in the plasma. The tissue expressions of MMP-8 in the surgically obtained samples were detected by Western blot and immunohistochemistry. Results The acute Stanford A aortic dissection patients showed a significantly higher level of MMP-8 in the plasma [ ( 106.51 ±28.27 ) ng/ml ] than the aortic valve replacement patients [ ( 25.65 ±12.95)ng/ml](P<0.05). Significant lesions of aortic intima, inflammatory cell infiltration laminated edge, elas-tic fibers and collagen fiber fragmentation, disintegration were observed in the experimental group, but not in the control group. The MMP-8 was significant expression in the experimental group than in the control group. Conclu-sion The MMP-8 is highly expressed in the middle of aortic lesions but lowly expressed in the normal fibrous tissue layer suggesting that it may play a role in development of the aortic disease.