安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
3期
327-329,330
,共4页
HCV RNA%T淋巴细胞亚群%相关性
HCV RNA%T淋巴細胞亞群%相關性
HCV RNA%T림파세포아군%상관성
HCV RNA%T cell subsets%correlation
目的探讨外周血中T淋巴细胞亚群与慢性丙型肝炎( CHC)患者丙型肝炎病毒( HCV)复制程度的关系。方法采用流式细胞仪直接免疫荧光法检测69例CHC患者、20例正常健康者外周血中的T细胞亚群；利用荧光定量PCR法检测患者血清HCV RNA。结果[1] CHC患者CD4+T细胞百分率[(42.87±6.11)]及 CD4+/CD8+比值[(1.34±0.25)]明显低于正常健康者[(49.55±6.68)]和[(1.82±0.11)](P<0.01),而CD8+T细胞百分率[(32.78±5.48)]则高于正常健康者[(27.35±4.32)](P<0.01),CD3+T细胞百分率差异无统计学意义(P>0.05)。于随着HCV RNA复制载量的增加,CD8+ T细胞百分率逐渐增加,而CD4+ T细胞百分率及CD4+/CD8+比值逐渐降低。结论 CHC患者外周T淋巴细胞亚群比例改变,可能是HCV感染慢性化的重要原因之一。随着HCV RNA表达水平的升高,可能是导致T淋巴细胞应答能力下降的重要原因。
目的探討外週血中T淋巴細胞亞群與慢性丙型肝炎( CHC)患者丙型肝炎病毒( HCV)複製程度的關繫。方法採用流式細胞儀直接免疫熒光法檢測69例CHC患者、20例正常健康者外週血中的T細胞亞群；利用熒光定量PCR法檢測患者血清HCV RNA。結果[1] CHC患者CD4+T細胞百分率[(42.87±6.11)]及 CD4+/CD8+比值[(1.34±0.25)]明顯低于正常健康者[(49.55±6.68)]和[(1.82±0.11)](P<0.01),而CD8+T細胞百分率[(32.78±5.48)]則高于正常健康者[(27.35±4.32)](P<0.01),CD3+T細胞百分率差異無統計學意義(P>0.05)。于隨著HCV RNA複製載量的增加,CD8+ T細胞百分率逐漸增加,而CD4+ T細胞百分率及CD4+/CD8+比值逐漸降低。結論 CHC患者外週T淋巴細胞亞群比例改變,可能是HCV感染慢性化的重要原因之一。隨著HCV RNA錶達水平的升高,可能是導緻T淋巴細胞應答能力下降的重要原因。
목적탐토외주혈중T림파세포아군여만성병형간염( CHC)환자병형간염병독( HCV)복제정도적관계。방법채용류식세포의직접면역형광법검측69례CHC환자、20례정상건강자외주혈중적T세포아군；이용형광정량PCR법검측환자혈청HCV RNA。결과[1] CHC환자CD4+T세포백분솔[(42.87±6.11)]급 CD4+/CD8+비치[(1.34±0.25)]명현저우정상건강자[(49.55±6.68)]화[(1.82±0.11)](P<0.01),이CD8+T세포백분솔[(32.78±5.48)]칙고우정상건강자[(27.35±4.32)](P<0.01),CD3+T세포백분솔차이무통계학의의(P>0.05)。우수착HCV RNA복제재량적증가,CD8+ T세포백분솔축점증가,이CD4+ T세포백분솔급CD4+/CD8+비치축점강저。결론 CHC환자외주T림파세포아군비례개변,가능시HCV감염만성화적중요원인지일。수착HCV RNA표체수평적승고,가능시도치T림파세포응답능력하강적중요원인。
Objective To investigate the correlation between T cell subsets and HCV viralload in HCV infection. Methods Flow cytometry was used to detect peripheral T cell subsets count of 69 patients with hepatitis C and 20 cases of normal health human, and fluorescent quantitative PCR was used to detect viral load in patients with hepa-titis C. Results ① The study showed that the percentage ratio of CD4 +T cells (42.87 ±6.11) and of CD4 +/CD8 +(1.34±0.25) in patients patients was significantly lower than those of normal health group,it was 49.55±6.68 and 1.82±0.11 (P<0.01);but the percentage ratio of CD8 +T cells (32.78±5.48) was higher than that of the normal health human ( 27.35 ±4.32 ) ( P<0.01 ) . There were no significant differences between the two groups in the percentage ratio of CD3 + T cells. ② The viral load gradually increased as the percentage ratio of CD8 +T cells increased, while the percentage ratio of CD4 +T cells and the percentage ratio of CD4 +T cells and CD4 +/ CD8 + were decreased gradually. Conclusion It may be one of the important reasons as the chronic infec-tion of hepatitis C virus by change of T cell subsets, and may be an important cause leading to the decrease of T lymphocyte response ability as the expression level of HCV RNA increases.
