安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
3期
295-299
,共5页
耿英华%武文娟%于北凯%夏瑞祥
耿英華%武文娟%于北凱%夏瑞祥
경영화%무문연%우북개%하서상
LY294002%PI3K/Akt%Skp2%K562
LY294002%PI3K/Akt%Skp2%K562
LY294002%PI3K/Akt%Skp2%K562
LY294002%PI3 K/Akt%Skp2%K562
目的探讨PI3K/Akt抑制剂LY294002对慢性髓系白血病细胞株K562的增殖抑制作用及相关机制。方法MTT法检测LY294002对K562细胞增殖的抑制作用;10、20μmol/L LY294002作用K562细胞36 h,流式细胞术观察细胞周期的变化, RT-PCR 法测定 LY294002对 K562细胞Skp2基因表达的影响,Western blot法检测Skp2蛋白表达的变化。结果 LY294002能够抑制K562细胞的生长,该抑制作用具有浓度及时间依赖性( P <0.05)。 LY294002作用K562细胞36 h,随着浓度的增加,G0/G1期阻滞增强,S期细胞减少(P<0.05),Skp2的mRNA表达量减少,Skp2蛋白的表达量显著降低。结论 LY294002能够抑制K562细胞的生长,诱导细胞发生 G0/G1期阻滞,这可能是通过LY294002影响了Skp2表达而实现的。
目的探討PI3K/Akt抑製劑LY294002對慢性髓繫白血病細胞株K562的增殖抑製作用及相關機製。方法MTT法檢測LY294002對K562細胞增殖的抑製作用;10、20μmol/L LY294002作用K562細胞36 h,流式細胞術觀察細胞週期的變化, RT-PCR 法測定 LY294002對 K562細胞Skp2基因錶達的影響,Western blot法檢測Skp2蛋白錶達的變化。結果 LY294002能夠抑製K562細胞的生長,該抑製作用具有濃度及時間依賴性( P <0.05)。 LY294002作用K562細胞36 h,隨著濃度的增加,G0/G1期阻滯增彊,S期細胞減少(P<0.05),Skp2的mRNA錶達量減少,Skp2蛋白的錶達量顯著降低。結論 LY294002能夠抑製K562細胞的生長,誘導細胞髮生 G0/G1期阻滯,這可能是通過LY294002影響瞭Skp2錶達而實現的。
목적탐토PI3K/Akt억제제LY294002대만성수계백혈병세포주K562적증식억제작용급상관궤제。방법MTT법검측LY294002대K562세포증식적억제작용;10、20μmol/L LY294002작용K562세포36 h,류식세포술관찰세포주기적변화, RT-PCR 법측정 LY294002대 K562세포Skp2기인표체적영향,Western blot법검측Skp2단백표체적변화。결과 LY294002능구억제K562세포적생장,해억제작용구유농도급시간의뢰성( P <0.05)。 LY294002작용K562세포36 h,수착농도적증가,G0/G1기조체증강,S기세포감소(P<0.05),Skp2적mRNA표체량감소,Skp2단백적표체량현저강저。결론 LY294002능구억제K562세포적생장,유도세포발생 G0/G1기조체,저가능시통과LY294002영향료Skp2표체이실현적。
Objective To explore the inhibition effects of LY294002(PI3K/Akt inhibition)on the proliferation and correlated regulatory factors of chronicmyeloid leukemia cell line K562 . Methods The effect of LY294002 on the proliferation of K562 cells were evaluated by MTT assays. K562 cells were cultured with 10,20 μmol/L LY294002 for 36 h. Flow cytometry analysis was used to determine the cell cycle. RT-PCR was applied to measure the expres-sion of S phase kinase associated protein 2 (Skp2) mRNA. Western blot was used to analyze Skp2 phenotypes. Re-sults Administered with LY294002,the proliferation of K562 cells were inhibited, the inhibition was in dose and time dependent manners(P<0.05). The G0/G1 period rate of K562 cells gradually raised and S period obviously decreased(P<0.05). The expression of Skp2 mRNA down-regulated, after K562 cells treated by LY294002 for 36 h. Compared with normal control group, the expression of Skp2 protein was significantly lower. ConclusionLY294002 can inhibit proliferation of K562 cells, induce G0/G1 period arrest,which possibly through the regula-tion of Skp2 expressions.