CAJ | 학술논문

目的记录大鼠心肌细胞生物电活动并观察天麻素对大鼠心肌细胞生物电活动的影响。方法选用健康 SD大鼠,应用心肌细胞内生物电记录的方法,了解正常窦房结及心房肌细胞的生物电活动情况,观察并分析天麻素对大鼠窦房结和心房肌细胞生物电活动的影响。结果在目前实验条件下,能真实记录到窦房结和心房肌细胞电活动,在此基础上观察到天麻素能加快大鼠窦房结细胞Vmax 4,缩短心房肌细胞APD90,其余的电生理参数变化不明显。结论记录离体心肌细胞生物电活动的实验条件相对成熟；天麻素能影响离体心肌细胞生物电活动,可能与天麻素增强 T-Ca2+通道通透性,促进KATP的开放有关。
목적기록대서심기세포생물전활동병관찰천마소대대서심기세포생물전활동적영향。방법선용건강 SD대서,응용심기세포내생물전기록적방법,료해정상두방결급심방기세포적생물전활동정황,관찰병분석천마소대대서두방결화심방기세포생물전활동적영향。결과재목전실험조건하,능진실기록도두방결화심방기세포전활동,재차기출상관찰도천마소능가쾌대서두방결세포Vmax 4,축단심방기세포APD90,기여적전생리삼수변화불명현。결론기록리체심기세포생물전활동적실험조건상대성숙；천마소능영향리체심기세포생물전활동,가능여천마소증강 T-Ca2+통도통투성,촉진KATP적개방유관。
Objective To record the myocardial cell bioelectric activities and explore electrophysiological profiles of Gastrodin on rat myocardial cells. Methods Sinoatrial node cells and atrial myocytes isolated from 50 SD rats were used in this study to characterize the effect of Gastrodin on their electrophysiological profiles with intracellular recording approaches. Results In the present experimental conditions, the bioelectric activities of sinoatrial node and atrial myocytes were truly recorded and we found that Gastrodin significantly accelerated AP frequency and Vmax 4 of sinoatrial node cells and shortened APD90 of atrial myocytes. Conclusion The experimental conditions of recording the bioelectric activities of myocardial cells in vitro are relatively mature. Gastrodin can affect the elec-trical activity of myocardial cells in vitro and the effects of Gastrodin may be related to inhance the permeability of T-Ca2+ channels and promote the opening of KATP.