CAJ | 학술논문

背景：前期实验发现，八肽胆囊收缩素能促进大鼠坐骨神经损伤后的再生，但具体机制仍不清楚。 　　目的：筛选有效指标，尝试从神经生长因子及神经再生微环境的角度分析八肽胆囊收缩素促进大鼠坐骨神经再生的机制。 　　方法：选择健康SD大鼠，制备坐骨神经单侧离断伤模型后随机分为2组，八肽胆囊收缩素治疗组造模后连续7 d腹腔注射八肽胆囊收缩素8 nmol/kg，对照组腹腔注射等量生理盐水。检测两组大鼠局部神经生长因子蛋白表达、脊髓诱导型一氧化氮合酶水平、血清超氧化物歧化酶活性和丙二醛浓度，同时检测脊髓凋亡细胞数。 　　结果与结论：八肽胆囊收缩素治疗组大鼠局部神经生长因子蛋白表达高于对照组(P <0.01)，脊髓诱导型一氧化氮合酶和凋亡细胞数低于对照组(P <0.01)，血清超氧化物歧化酶活性高于对照组且丙二醛浓度低于对照组(P<0.01，0.05)。说明胆囊收缩素促进坐骨神经再生的可能机制包括保护神经元、抗细胞凋亡、抑制炎性反应、抗NO及氧化反应、抗丙二醛减轻自由基损伤等方面外，还可刺激神经生长因子的表达和释放。
배경：전기실험발현，팔태담낭수축소능촉진대서좌골신경손상후적재생，단구체궤제잉불청초。 　　목적：사선유효지표，상시종신경생장인자급신경재생미배경적각도분석팔태담낭수축소촉진대서좌골신경재생적궤제。 　　방법：선택건강SD대서，제비좌골신경단측리단상모형후수궤분위2조，팔태담낭수축소치료조조모후련속7 d복강주사팔태담낭수축소8 nmol/kg，대조조복강주사등량생리염수。검측량조대서국부신경생장인자단백표체、척수유도형일양화담합매수평、혈청초양화물기화매활성화병이철농도，동시검측척수조망세포수。 　　결과여결론：팔태담낭수축소치료조대서국부신경생장인자단백표체고우대조조(P <0.01)，척수유도형일양화담합매화조망세포수저우대조조(P <0.01)，혈청초양화물기화매활성고우대조조차병이철농도저우대조조(P<0.01，0.05)。설명담낭수축소촉진좌골신경재생적가능궤제포괄보호신경원、항세포조망、억제염성반응、항NO급양화반응、항병이철감경자유기손상등방면외，환가자격신경생장인자적표체화석방。
BACKGROUND:Previous studies have found that cholecystokinin octapeptide (CCK-8) can promote the regeneration after sciatic nerve injury in rats, but the exact mechanism remains unclear. OBJECTIVE:To screen effective indicators and analyze the mechanism of CCK-8 promoting sciatic nerve regeneration from the perspective of nerve growth factor and nerve regeneration microenvironment. METHODS:Healthy Sprague-Dawley rats, for the preparation of unilateral sciatic nerve transection injury model, were randomly divided into two groups. In the CCK-8 group, the animal model received intraperitoneal injection of CCK-8 (8 nmol/kg) for consecutive 7 days, while the control group was injected with equal volume of normal saline. The nerve growth factor expression, inducible nitric oxide synthase in the spinal cord, serum superoxide dismutase activity and malondialdehyde concentration, as wel as apoptotic cel s in spinal cord were al detected. RESULTS AND CONCLUSION:In the CCK-8 group, nerve growth factor expression was higher than that in the control group (P<0.01), while inducible nitric oxide synthase and the number of apoptotic cel s were lower (P<0.01), serum superoxide dismutase activity was higher but malondialdehyde concentrations was lower (P<0.01, 0.05). The mechanisms of CCK-8 promoting sciatic nerve regeneration include protecting neurons, anti-apoptosis, inhibiting inflammatory response, anti-NO and anti-oxidation, reducing malondialdehyde, and al eviating free radical damage, as wel as stimulating nerve growth factor expression and release.