CAJ | 학술논문

背景：体外冲击波等应力刺激可促进成骨，甲状旁腺激素激素也参与调控骨代谢。 　　目的：实验探讨低剂量间歇人重组甲状旁腺素1-34和低能体外冲击波对体外培养大鼠成骨细胞增殖及成骨分化的作用。方法：采用改良胶原酶消化法培养大鼠乳鼠颅骨来源成骨细胞备用。分别用60-150次0.18 mJ/mm2低能体外冲击波刺激体外培养大鼠成骨细胞，不同浓度(10-12 mol/L-10-10 mol/L)及作用方式的人重组甲状旁腺素1-34刺激，以及低能体外冲击波和间歇低剂量(10-11 mol/L)间歇人重组甲状旁腺素1-34刺激共同作用后，用锥虫蓝法进行细胞计数、MTT和流式细胞术分析检测大鼠成骨细胞的增殖情况；用酶标仪检测碱性磷酸酶活性，用免疫组化检测Ⅰ型胶原表达来观察大鼠成骨细胞的成骨分化。 　　结果与结论：60-150次0.18 mJ/mm 2低能体外冲击波刺激、间歇人重组甲状旁腺素1-34(10-11和10-10 mol/L)刺激以及低能体外冲击波+间歇人重组甲状旁腺素1-34(10-11 mol/L)刺激均可显著促进体外培养大鼠成骨细胞增殖和成骨分化(P<0.05)，其中60-150次低能体外冲击波刺激+间歇人重组甲状旁腺素1-34刺激各组作用最强(P <0.05)。结果证实，适当的低能体外冲击波应力刺激和低剂量间歇人重组甲状旁腺素1-34刺激联合应用可显著促进体外培养大鼠成骨细胞的增殖和成骨分化。
배경：체외충격파등응력자격가촉진성골，갑상방선격소격소야삼여조공골대사。 　　목적：실험탐토저제량간헐인중조갑상방선소1-34화저능체외충격파대체외배양대서성골세포증식급성골분화적작용。방법：채용개량효원매소화법배양대서유서로골래원성골세포비용。분별용60-150차0.18 mJ/mm2저능체외충격파자격체외배양대서성골세포，불동농도(10-12 mol/L-10-10 mol/L)급작용방식적인중조갑상방선소1-34자격，이급저능체외충격파화간헐저제량(10-11 mol/L)간헐인중조갑상방선소1-34자격공동작용후，용추충람법진행세포계수、MTT화류식세포술분석검측대서성골세포적증식정황；용매표의검측감성린산매활성，용면역조화검측Ⅰ형효원표체래관찰대서성골세포적성골분화。 　　결과여결론：60-150차0.18 mJ/mm 2저능체외충격파자격、간헐인중조갑상방선소1-34(10-11화10-10 mol/L)자격이급저능체외충격파+간헐인중조갑상방선소1-34(10-11 mol/L)자격균가현저촉진체외배양대서성골세포증식화성골분화(P<0.05)，기중60-150차저능체외충격파자격+간헐인중조갑상방선소1-34자격각조작용최강(P <0.05)。결과증실，괄당적저능체외충격파응력자격화저제량간헐인중조갑상방선소1-34자격연합응용가현저촉진체외배양대서성골세포적증식화성골분화。
BACKGROUND:Suitable stress signals such as extracorporeal shock wave (ESW) and parathyroid hormone can be involved in regulation of bone formation and metabolism. OBJECTIVE:To investigate the effects of low-density ESW and low-dose intermittent recombinant human parathyroid hormone 1-34 (rhPTH1-34) on the proliferation and differentiation of osteoblasts from rats cultured in vitro. METHODS:Osteoblasts from the skul of neonatal rats were cultured using col agenase digestion method. After 60-150 times of stimulations of 0.18 mJ/mm2 ESW, different patterns of rhPTH1-34 at 10-12-10-10 mol/L and ESW+intermittent rhPTH1-34 (10-11 mol/L), osteoblasts were col ected to observe cel proliferation by cel counting, MTT and flow cytometry analysis and to observe cel differentiation by measuring alkaline phosphatase and type I col agen expression. RESULTS AND CONCLUSION:0.18 mJ/mm 2 ESW (60-150 times), intermittent rhPTH1-34 (10-11 and 10-10 mol/L) and ESW+intermittent rhPTH1-34 (10-11 mol/L) stimulations could al improve proliferation and differentiation of rat osteoblasts cultured in vitro (P<0.05). ESW (60-150 times)+intermittent rhPTH1-34 stimulation showed better effects than ESW and intermittent rhPTH1-34 alone (P<0.05). These findings indicate that the combination of suitable ESW stimulation and low-dose intermittent rhPTH1-34 stimulation can improve the proliferation and differentiation of rat osteoblasts significantly.