中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
11期
1659-1664
,共6页
周智%刘开祥%谢跃%王黎明
週智%劉開祥%謝躍%王黎明
주지%류개상%사약%왕려명
关节炎, 类风湿%硫化氢%肿瘤坏死因子α%白细胞介素1β%胶原Ⅱ型%滑膜%软骨
關節炎, 類風濕%硫化氫%腫瘤壞死因子α%白細胞介素1β%膠原Ⅱ型%滑膜%軟骨
관절염, 류풍습%류화경%종류배사인자α%백세포개소1β%효원Ⅱ형%활막%연골
arthritis,rheumatoid%hydrogen sulfide%tumor necrosis factor-alpha%interleukin-1 beta%col agen type II%synovial membrane%cartilage
背景：类风湿性关节炎是一种常见的慢性系统性自身免疫疾病，以关节滑膜病变、骨质侵蚀为主要特征，临床上的治疗仍然比较棘手。<br> 目的：探讨外源性硫化氢对类风湿性关节炎大鼠关节病变的影响。<br> 方法：将SD大鼠分为4组：空白组、模型组、高浓度硫化氢干预组、低浓度硫化氢干预组。干预2，4周时，测量关节炎指数，血清炎症因子肿瘤坏死因子α和白细胞介素1β水平，滑膜组织病理学变化，膝关节标本Ⅱ型胶原染色的平均吸光度值。<br> 结果与结论：从关节炎指数，血清炎症因子水平以及滑膜染色炎性浸润方面看，模型组以及硫化氢干预组均高于空白组，高浓度硫化氢干预组明显高于模型组(P <0.05)，低浓度硫化氢干预组低于模型组(P <0.05)。在膝关节软骨Ⅱ型胶原染色的平均吸光度值方面，模型组以及2个硫化氢干预组均低于空白组，高浓度硫化氢干预组明显低于模型组(P <0.05)，低浓度硫化氢干预组高于模型组(P <0.05)。结果可见硫化氢对类风湿关节炎症有调节作用，高浓度的硫化氢加重关节炎症及软骨降解，低浓度的硫化氢减轻关节炎症并对软骨有保护作用。
揹景：類風濕性關節炎是一種常見的慢性繫統性自身免疫疾病，以關節滑膜病變、骨質侵蝕為主要特徵，臨床上的治療仍然比較棘手。<br> 目的：探討外源性硫化氫對類風濕性關節炎大鼠關節病變的影響。<br> 方法：將SD大鼠分為4組：空白組、模型組、高濃度硫化氫榦預組、低濃度硫化氫榦預組。榦預2，4週時，測量關節炎指數，血清炎癥因子腫瘤壞死因子α和白細胞介素1β水平，滑膜組織病理學變化，膝關節標本Ⅱ型膠原染色的平均吸光度值。<br> 結果與結論：從關節炎指數，血清炎癥因子水平以及滑膜染色炎性浸潤方麵看，模型組以及硫化氫榦預組均高于空白組，高濃度硫化氫榦預組明顯高于模型組(P <0.05)，低濃度硫化氫榦預組低于模型組(P <0.05)。在膝關節軟骨Ⅱ型膠原染色的平均吸光度值方麵，模型組以及2箇硫化氫榦預組均低于空白組，高濃度硫化氫榦預組明顯低于模型組(P <0.05)，低濃度硫化氫榦預組高于模型組(P <0.05)。結果可見硫化氫對類風濕關節炎癥有調節作用，高濃度的硫化氫加重關節炎癥及軟骨降解，低濃度的硫化氫減輕關節炎癥併對軟骨有保護作用。
배경：류풍습성관절염시일충상견적만성계통성자신면역질병，이관절활막병변、골질침식위주요특정，림상상적치료잉연비교극수。<br> 목적：탐토외원성류화경대류풍습성관절염대서관절병변적영향。<br> 방법：장SD대서분위4조：공백조、모형조、고농도류화경간예조、저농도류화경간예조。간예2，4주시，측량관절염지수，혈청염증인자종류배사인자α화백세포개소1β수평，활막조직병이학변화，슬관절표본Ⅱ형효원염색적평균흡광도치。<br> 결과여결론：종관절염지수，혈청염증인자수평이급활막염색염성침윤방면간，모형조이급류화경간예조균고우공백조，고농도류화경간예조명현고우모형조(P <0.05)，저농도류화경간예조저우모형조(P <0.05)。재슬관절연골Ⅱ형효원염색적평균흡광도치방면，모형조이급2개류화경간예조균저우공백조，고농도류화경간예조명현저우모형조(P <0.05)，저농도류화경간예조고우모형조(P <0.05)。결과가견류화경대류풍습관절염증유조절작용，고농도적류화경가중관절염증급연골강해，저농도적류화경감경관절염증병대연골유보호작용。
BACKGROUND:Rheumatoid arthritis is a common chronic systemic autoimmune disease which is characterized by synovial lesions and bone erosion. The clinical treatment is stil a difficulty. <br> OBJECTIVE:To explore the effect of exogenous hydrogen sulfide on the articular lesions in rheumatoid arthritis rats. <br> METHODS:Sprague-Dawley rats were divided into four groups:control group, model group, high concentrations of hydrogen sulfide intervention group, low concentrations of hydrogen sulfide intervention group. At 2 and 4 weeks after intervention, the arthritis index, levels of serum inflammatory cytokines (tumor necrotic factor-αand interleukin-1β), pathological change of synovial tissue, average absorbance of knee joint specimens using col agen-II immunohistochemical staining were measured. <br> RESULTS AND CONCLUSION:The arthritis index, levels of serum inflammatory cytokines and inflammatory infiltration of synovial staining in the model group and two intervention groups were higher than that in control group, the high concentrations of hydrogen sulfide intervention group was significantly higher than the model group (P<0.05) while the low concentrations of hydrogen sulfide intervention group was lower than the model group (P<0.05). The average absorbance of knee cartilage specimens using col agen-II immunohistochemical staining was lower in the model group and two hydrogen sulfide intervention groups compared with the control group, the high concentrations of hydrogen sulfide intervention group was significantly lower than the model group (P<0.05) while low concentrations of hydrogen sulfide intervention group was higher than the model group (P<0.05). Hydrogen sulfide has a regulatory effect on rheumatoid arthritis, high concentrations of hydrogen sulfide aggravate arthritis and cartilage degradation, low concentrations of hydrogen sulfide reduce arthritis and protect cartilage.
