中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
11期
1653-1658
,共6页
杨颖%仲维剑%刘国%马国武
楊穎%仲維劍%劉國%馬國武
양영%중유검%류국%마국무
组织构建%骨组织构建%种植体%骨缺损%骨髓浓缩物%骨再生
組織構建%骨組織構建%種植體%骨缺損%骨髓濃縮物%骨再生
조직구건%골조직구건%충식체%골결손%골수농축물%골재생
dental implants%bone marrow%bone regeneration%gelatin sponge,absorbable
背景：目前，自体骨髓浓缩物主要应用于骨科、重症肢体缺血患者，其在口腔颌面外科尤其是种植外科应用较少，在种植体周围促进新骨再生的效果也有待研究。<br> 目的：评价自体骨髓浓缩物在种植体周围骨缺损修复中的作用。<br> 方法：于实验犬髂后上棘处抽取骨髓5 mL，对浓缩前后的骨髓进行细胞计数。在实验犬的双侧下颌前磨牙区植入种植体，并于近中制作4 mm×4 mm×4 mm的骨缺损，随机植入骨髓浓缩物混合明胶海绵、自体骨、明胶海绵。术后4，12周对骨缺损标本进行组织学观察分析，并计算新骨生成率和新骨密度。<br> 结果与结论：骨髓细胞平均浓缩倍数为(2.78±0.22)倍。浓缩骨髓中，可观察到更多和更大的克隆单位。组织学分析发现，4周时骨髓浓缩物明胶海绵组的新骨生成率和密度均明显高于自体骨组和明胶海绵组(P<0.05)，12周时骨髓浓缩物明胶海绵组新骨生成率明显低于自体骨组，但高于明胶海绵组(P<0.05)，3组新骨密度差异无显著性意义(P>0.05)。结果显示自体骨髓浓缩物能够显著提高种植体周围骨缺损内早期新生骨的质量。
揹景：目前，自體骨髓濃縮物主要應用于骨科、重癥肢體缺血患者，其在口腔頜麵外科尤其是種植外科應用較少，在種植體週圍促進新骨再生的效果也有待研究。<br> 目的：評價自體骨髓濃縮物在種植體週圍骨缺損脩複中的作用。<br> 方法：于實驗犬髂後上棘處抽取骨髓5 mL，對濃縮前後的骨髓進行細胞計數。在實驗犬的雙側下頜前磨牙區植入種植體，併于近中製作4 mm×4 mm×4 mm的骨缺損，隨機植入骨髓濃縮物混閤明膠海綿、自體骨、明膠海綿。術後4，12週對骨缺損標本進行組織學觀察分析，併計算新骨生成率和新骨密度。<br> 結果與結論：骨髓細胞平均濃縮倍數為(2.78±0.22)倍。濃縮骨髓中，可觀察到更多和更大的剋隆單位。組織學分析髮現，4週時骨髓濃縮物明膠海綿組的新骨生成率和密度均明顯高于自體骨組和明膠海綿組(P<0.05)，12週時骨髓濃縮物明膠海綿組新骨生成率明顯低于自體骨組，但高于明膠海綿組(P<0.05)，3組新骨密度差異無顯著性意義(P>0.05)。結果顯示自體骨髓濃縮物能夠顯著提高種植體週圍骨缺損內早期新生骨的質量。
배경：목전，자체골수농축물주요응용우골과、중증지체결혈환자，기재구강합면외과우기시충식외과응용교소，재충식체주위촉진신골재생적효과야유대연구。<br> 목적：평개자체골수농축물재충식체주위골결손수복중적작용。<br> 방법：우실험견가후상극처추취골수5 mL，대농축전후적골수진행세포계수。재실험견적쌍측하합전마아구식입충식체，병우근중제작4 mm×4 mm×4 mm적골결손，수궤식입골수농축물혼합명효해면、자체골、명효해면。술후4，12주대골결손표본진행조직학관찰분석，병계산신골생성솔화신골밀도。<br> 결과여결론：골수세포평균농축배수위(2.78±0.22)배。농축골수중，가관찰도경다화경대적극륭단위。조직학분석발현，4주시골수농축물명효해면조적신골생성솔화밀도균명현고우자체골조화명효해면조(P<0.05)，12주시골수농축물명효해면조신골생성솔명현저우자체골조，단고우명효해면조(P<0.05)，3조신골밀도차이무현저성의의(P>0.05)。결과현시자체골수농축물능구현저제고충식체주위골결손내조기신생골적질량。
BACKGROUND:Autologous bone marrow aspirate concentrate is often applied in patients from Department of Orthopedics and those with severe limb ischemia, but rarely applied in Department of Oral and Maxil ofacial Surgery, especial y in Department of Oral Implantology. The effect of autologous bone marrow aspirate concentrate on promoting peri-implant bone regeneration deserves further studies. <br> OBJECTIVE:To evaluate the effect of bone marrow aspirate concentrate in the repair of peri-implant bone defect. <br> METHODS:Bone marrow 5 mL was extracted from posterior superior iliac spine of experimental dogs and bone marrow cel s were counted before and after concentration. Bone defect (4 mm × 4 mm × 4 mm) was prepared in the middle of bilateral mandibular premolar, which was randomly implanted with gelatin sponge plus bone marrow aspirate concentrate, autologous bone and gelatin sponge. At 4 and 12 weeks after surgery, bone defect specimens were histological y observed. The new bone formation rate and new bone mineral density were calculated. <br> RESULTS AND CONCLUSION:After centrifugation, the concentrations of nucleated cel s in bone marrow aspirate concentrate were increased by (2.78±0.22) times. More colony-forming units were found after cel culture. Histological analysis showed that, significantly higher new bone formation rate and new bone mineral density occurred in gelatin sponge plus bone marrow aspirate concentrate group, compared with autologous bone group and gelatin sponge group at 4 weeks (P<0.05). The new bone formation rate in gelatin sponge plus bone marrow aspirate concentrate group was significantly lower than that of autologous bone group, and higher than that of gelatin sponge group at 12 weeks (P<0.05). However, the difference of new bone mineral density in the three groups was not significant (P>0.05). Autologous bone marrow aspirate concentrate can significantly improve new bone mineral density and quantity in the pre-implant bone defect.
