CAJ | 학술논문

生物材料%材料生物相容性%聚羟基丁酸/戊酸酯共聚物%人骨髓间充质干细胞%生物相容性%组织工程%细胞贴壁率%细胞增殖%体外%DNA含量%高分子%材料活性%国家自然科学基金生物材料%材料生物相容性%聚羥基丁痠/戊痠酯共聚物%人骨髓間充質榦細胞%生物相容性%組織工程%細胞貼壁率%細胞增殖%體外%DNA含量%高分子%材料活性%國傢自然科學基金
생물재료%재료생물상용성%취간기정산/무산지공취물%인골수간충질간세포%생물상용성%조직공정%세포첩벽솔%세포증식%체외%DNA함량%고분자%재료활성%국가자연과학기금
biocompatible materials%stem cel s%3-hydroxybutyric acid%histocompatibility

背景：聚羟基丁酸/戊酸酯共聚物是近年来受到重视的聚羟基脂肪酸族组织工程支架材料，具有免疫排斥反应低、生物相容性好和降解产物无毒副作用的优点。 　　目的：观察聚羟基丁酸/戊酸酯共聚物膜材料与人骨髓间充质干细胞的体外生物相容性。 　　方法：将第3代人骨髓间充质干细胞种植于聚羟基丁酸/戊酸酯共聚物膜上作为实验组，培养板单纯培养细胞作为对照组，计算1，2，4 h两组贴壁细胞的数量，得出细胞贴壁率。MTT比色法观察2，4，6，8 d两组细胞的增殖情况。采用Hoechst33258荧光法，检测3，6，9，12 d两组细胞内的DNA含量。将人骨髓间充质干细胞接种聚羟基丁酸/戊酸酯共聚物膜材料上5d后，电镜扫描观察细胞在材料上的生长情况。 　　结果与结论：共培养1h时，实验组的细胞贴壁率低于对照组；其他时间段两组之间细胞贴壁率差异无显著意义。两组各时点间的细胞增殖差异无显著意义。两组各时间点细胞内DNA含量差异无显著意义。扫描电镜观察人骨髓间充质干细胞在聚羟基丁酸/戊酸酯共聚物膜上生长良好，形态呈梭形，细胞间连接紧密，分泌较多细胞基质。证明聚羟基丁酸/戊酸酯共聚物膜材料与人骨髓间充质干细胞有良好的生物相容性。
배경：취간기정산/무산지공취물시근년래수도중시적취간기지방산족조직공정지가재료，구유면역배척반응저、생물상용성호화강해산물무독부작용적우점。 　　목적：관찰취간기정산/무산지공취물막재료여인골수간충질간세포적체외생물상용성。 　　방법：장제3대인골수간충질간세포충식우취간기정산/무산지공취물막상작위실험조，배양판단순배양세포작위대조조，계산1，2，4 h량조첩벽세포적수량，득출세포첩벽솔。MTT비색법관찰2，4，6，8 d량조세포적증식정황。채용Hoechst33258형광법，검측3，6，9，12 d량조세포내적DNA함량。장인골수간충질간세포접충취간기정산/무산지공취물막재료상5d후，전경소묘관찰세포재재료상적생장정황。 　　결과여결론：공배양1h시，실험조적세포첩벽솔저우대조조；기타시간단량조지간세포첩벽솔차이무현저의의。량조각시점간적세포증식차이무현저의의。량조각시간점세포내DNA함량차이무현저의의。소묘전경관찰인골수간충질간세포재취간기정산/무산지공취물막상생장량호，형태정사형，세포간련접긴밀，분비교다세포기질。증명취간기정산/무산지공취물막재료여인골수간충질간세포유량호적생물상용성。
BACKGROUND:Polyhydroxybutyrate-co-volerate (PHBV) is a noticeable tissue engineering material of polyhydroxyalkanoates family. It has the properties of low immune rejection response and good biocompatibility, and its degradation products are non-toxic. OBJECTIVE:To investigate the biocompatibility of PHBV membrane material and human bone marrow mesenchymal stem cel s in vitro. METHODS:Human bone marrow mesenchymal stem cel s at passage 3 were seeded upon PHBV membrane as experimental group and upon conventional culture plates as control group. Then we calculated the adherent cel number of two groups at 1, 2 and 4 hours and got the cel adherent rate. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay was used at days 2, 4, 6, 8 to observe the cel proliferation of two groups. Fluorimetric method with the fluorescent dye Hoechst 33258 was used to detect the DNA content of cel s at days 3, 6, 9 and 12 in both groups. After cel s were seeded upon PHBV membrane for 5 days, the cel growth upon the material was examined under a scanning electron microscope. RESULTS AND CONCLUSION:When the cel s were cultured for 1 hour, the adherent rate in the experimental group was lower than that in the control group;but there were no significant differences between two groups at the other two periods. No difference was found in the cel proliferation and the DNA content between the two groups. Human bone marrow mesenchymal stem cel s seeded upon PHBV membrane for 5 days grew wel with spindle morphology and the intercel ular connections were tight and more extracel ular matrices were observed by scanning electron microscopy. Taken together, PHBV membrane material shows a good biocompatibility with human bone marrow mesenchymal stem cel s.