中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
12期
1901-1906
,共6页
生物材料%口腔生物材料%羟基磷灰石%含氟羟基磷灰石%种植体%生物相容性%纳米材料
生物材料%口腔生物材料%羥基燐灰石%含氟羥基燐灰石%種植體%生物相容性%納米材料
생물재료%구강생물재료%간기린회석%함불간기린회석%충식체%생물상용성%납미재료
biocompatible materials%hydroxyapatites%dental implants
背景：有关纳米含氟羟基磷灰石牙种植体材料生物相容性的报道较少。<br> 目的：检测纳米含氟羟基磷灰石牙种植体材料的体外生物相容性。<br> 方法：采用溶胶凝胶技术分别制备羟基磷灰石与纳米含氟羟基磷灰石。①溶血性实验：在0.2 mL稀释兔抗凝血中分别加入0.01，0.15，0.2 g/L纳米含氟羟基磷灰石溶液、生理盐水及蒸馏水各10 mL，检测各组上清液吸光度值。②体外细胞毒性实验：分别以100%，50%纳米含氟羟基磷灰石浸提液、100%羟基磷灰石浸提液、苯酚溶液及RPMI1640培养液培养传至第2代的L929细胞，MTT法检测培养2，4，7 d的吸光度值。<br> 结果与结论：体外溶血性实验显示，各浓度梯度纳米含氟羟基磷灰石的溶血率均在5%以内，符合医用材料的溶血要求。体外细胞毒性实验显示，随着培养时间的增加，100%，50%纳米含氟羟基磷灰石浸提液组细胞贴壁覆盖率增加，细胞密度增高，细胞为长梭形或多角形，细胞增殖及形态与RPMI1640培养液组、羟基磷灰石组无明显差别，细胞毒性为0级。
揹景：有關納米含氟羥基燐灰石牙種植體材料生物相容性的報道較少。<br> 目的：檢測納米含氟羥基燐灰石牙種植體材料的體外生物相容性。<br> 方法：採用溶膠凝膠技術分彆製備羥基燐灰石與納米含氟羥基燐灰石。①溶血性實驗：在0.2 mL稀釋兔抗凝血中分彆加入0.01，0.15，0.2 g/L納米含氟羥基燐灰石溶液、生理鹽水及蒸餾水各10 mL，檢測各組上清液吸光度值。②體外細胞毒性實驗：分彆以100%，50%納米含氟羥基燐灰石浸提液、100%羥基燐灰石浸提液、苯酚溶液及RPMI1640培養液培養傳至第2代的L929細胞，MTT法檢測培養2，4，7 d的吸光度值。<br> 結果與結論：體外溶血性實驗顯示，各濃度梯度納米含氟羥基燐灰石的溶血率均在5%以內，符閤醫用材料的溶血要求。體外細胞毒性實驗顯示，隨著培養時間的增加，100%，50%納米含氟羥基燐灰石浸提液組細胞貼壁覆蓋率增加，細胞密度增高，細胞為長梭形或多角形，細胞增殖及形態與RPMI1640培養液組、羥基燐灰石組無明顯差彆，細胞毒性為0級。
배경：유관납미함불간기린회석아충식체재료생물상용성적보도교소。<br> 목적：검측납미함불간기린회석아충식체재료적체외생물상용성。<br> 방법：채용용효응효기술분별제비간기린회석여납미함불간기린회석。①용혈성실험：재0.2 mL희석토항응혈중분별가입0.01，0.15，0.2 g/L납미함불간기린회석용액、생리염수급증류수각10 mL，검측각조상청액흡광도치。②체외세포독성실험：분별이100%，50%납미함불간기린회석침제액、100%간기린회석침제액、분분용액급RPMI1640배양액배양전지제2대적L929세포，MTT법검측배양2，4，7 d적흡광도치。<br> 결과여결론：체외용혈성실험현시，각농도제도납미함불간기린회석적용혈솔균재5%이내，부합의용재료적용혈요구。체외세포독성실험현시，수착배양시간적증가，100%，50%납미함불간기린회석침제액조세포첩벽복개솔증가，세포밀도증고，세포위장사형혹다각형，세포증식급형태여RPMI1640배양액조、간기린회석조무명현차별，세포독성위0급。
BACKGROUND:Few reports focus on biocompatibility of nano fluoridated hydroxyapatite for dental implants. OBJECTIVE:To investigate the biocompatibility in vitro of nano fluoridated hydroxyapatite modified for dental implants. <br> METHODS:We utilized sol-gel method to prepare nano fluoridated hydroxyapatite and nanohydroxyapatite powders. (1) Hemolysis test:0.01, 0.15, 0.2 g/L nano fluorinated hydroxyapatite solution, saline and distil ed water at a volume of 10 mL were added into 0.2 mL diluted rabbit anti-coagulation blood samples, respectively. Then the supernatant was detected by absorbance values. (2) In vitro cytotoxicity test:Passage 2 L929 cel s were respectively cultured in culture media containing 100%, 50%nano fluorinated hydroxyapatite extract, 100%hydroxyapatite extract, phenol solution and RPMI1640. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was employed to measure absorbance values at days 2, 4, 7 of culture. <br> RESULTS AND CONCLUSION:Hemolysis test in vitro showed the hemolysis rates of nano fluorinated hydroxyapatite groups were less than 5%, which were accorded with haemolysis demand of medical materials. The cytotoxicity test in vitro showed during cultivation, the adherence rate of L929 cel s cultured in 100%and 50%nano fluorinated hydroxyapatite extracts were increasing and cel density was rising up. Cel s were fusiform or polygon, which had no evident differences from negative controls in morphology. Nano fluorinated hydroxyapatite showed nontoxic to L929 cel s in vitro.
