CAJ | 학술논문

背景：已有很多实验证明，单独高分子材料或生物性材料制备的组织工程支架无法满足组织工程研究。目的：评价羟基丁酸-羟基辛酸聚合物/胶原组织工程支架的生物学特性及细胞亲和性。 　　方法：以羟基丁酸-羟基辛酸聚合物作为主体材料，按质量分数复合不同比例(2%，4%，6%，8%，10%)的胶原，采用溶剂浇铸-颗粒沥滤法制备组织工程支架。通过扫描电镜观察材料内部结构及孔径大小，液体位移法测定材料孔隙率。将羟基丁酸-羟基辛酸聚合物/胶原支架、羟基丁酸-羟基辛酸聚合物支架分别与兔软骨细胞复合培养，MTT法测定细胞的生长曲线，扫描电镜观察细胞在材料上的生长黏附情况。 　　结果与结论：羟基丁酸-羟基辛酸聚合物/胶原复合软骨组织工程支架孔径大小200μm 左右，孔隙率为(85±2)%，细胞亲水性随加入胶原比例的增加而升高。与羟基丁酸-羟基辛酸聚合物支架比较，不同比例的羟基丁酸-羟基辛酸聚合物/胶原支架可明显促进软骨细胞的黏附、增殖。证实羟基丁酸-羟基辛酸聚合物/胶原复合支架具备更好的细胞亲和性。
배경：이유흔다실험증명，단독고분자재료혹생물성재료제비적조직공정지가무법만족조직공정연구。목적：평개간기정산-간기신산취합물/효원조직공정지가적생물학특성급세포친화성。 　　방법：이간기정산-간기신산취합물작위주체재료，안질량분수복합불동비례(2%，4%，6%，8%，10%)적효원，채용용제요주-과립력려법제비조직공정지가。통과소묘전경관찰재료내부결구급공경대소，액체위이법측정재료공극솔。장간기정산-간기신산취합물/효원지가、간기정산-간기신산취합물지가분별여토연골세포복합배양，MTT법측정세포적생장곡선，소묘전경관찰세포재재료상적생장점부정황。 　　결과여결론：간기정산-간기신산취합물/효원복합연골조직공정지가공경대소200μm 좌우，공극솔위(85±2)%，세포친수성수가입효원비례적증가이승고。여간기정산-간기신산취합물지가비교，불동비례적간기정산-간기신산취합물/효원지가가명현촉진연골세포적점부、증식。증실간기정산-간기신산취합물/효원복합지가구비경호적세포친화성。
BACKGROUND:Many experiments have demonstrated that tissue engineering scaffolds prepared by polymer materials alone or biomaterials cannot meet the requirement of tissue engineering research. OBJECTIVE:To evaluate biological characteristics and cel affinity of poly(hydroxybutyrate-co-hydroxyoctanoate)/col agen composite scaffold. METHODS:Tissue engineering scaffolds were prepared by combination of poly(hydroxybutyrate-co-hydroxyoctanoate) and col agen at different proportions (2%, 4%, 6%, 8%and 10%) using solvent casting/particulate leaching method. Inner structure and apertures were observed by scanning electron microscope, and the porosity was determined by liquid displacement method. Rabbit chondrocytes were co-cultured with poly(hydroxybutyrate-co-hydroxyoctanoate)/col agen composite scaffold and poly(hydroxybutyrate-co-hydroxyoctanoate) scaffold. Growth curve of cel s was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cel adhesion on the scaffolds was observed by scanning electron microscope. RESULTS AND CONCLUSION:The pore size and porosity of the composite scaffold were about 200μm and (85±2)%, respectively. The cel affinity dynamical y increased with the increasing of proportion of col agen. Compared with the poly(hydroxybutyrate-co-hydroxyoctanoate) scaffold, the poly(hydroxybutyrate-co-hydroxyoctanoate)/col agen composite scaffolds are better to improve cel adhesion and proliferation, with favorable cel ular affinity.