中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
11期
775-778
,共4页
胡燕荣%刘建勇%张德志%普雄明
鬍燕榮%劉建勇%張德誌%普雄明
호연영%류건용%장덕지%보웅명
银屑病%寡核苷酸序列分析%NF-κB%粒细胞巨噬细胞集落刺激因子%CARD11
銀屑病%寡覈苷痠序列分析%NF-κB%粒細胞巨噬細胞集落刺激因子%CARD11
은설병%과핵감산서렬분석%NF-κB%립세포거서세포집락자격인자%CARD11
Psoriasis%Oligonucleotide array sequence analysis%NF-kappaB%Granulocyte-macrophage colony-stimulating factor%CARD11
目的 检测维吾尔族银屑病患者皮损组织与核因子κB(NF-κB)相关的84个信号分子的表达,初步探讨表达明显异常的信号分子.方法 收集8例新疆维吾尔族银屑病患者皮损组织,同时收集患者的正常皮肤组织作为对照,提取组织总RNA,逆转录生成cDNA后,聚合酶链反应Array法(PCR-Array)检测NF-κB相关的84个信号分子表达水平,以2倍为上调或者下调的检验标准.结果 84种信号分子中,22种信号分子表达上调,7种下调.其中,上调最为明显的分子为胱冬肽酶募集结构域(CARD 11)及粒细胞巨噬细胞集落刺激因子(CSF2),下调最为明显的为肿瘤坏死因子超家族成员5(CD40)、白介素10(IL-10)及B细胞κ轻肽基因增强子核因子抑制因子ε(NFκBIE).结论 在检测的银屑病患者皮损组织中,NF-κB信号通路相关的多种分子可能处于被激活状态或抑制状态.
目的 檢測維吾爾族銀屑病患者皮損組織與覈因子κB(NF-κB)相關的84箇信號分子的錶達,初步探討錶達明顯異常的信號分子.方法 收集8例新疆維吾爾族銀屑病患者皮損組織,同時收集患者的正常皮膚組織作為對照,提取組織總RNA,逆轉錄生成cDNA後,聚閤酶鏈反應Array法(PCR-Array)檢測NF-κB相關的84箇信號分子錶達水平,以2倍為上調或者下調的檢驗標準.結果 84種信號分子中,22種信號分子錶達上調,7種下調.其中,上調最為明顯的分子為胱鼕肽酶募集結構域(CARD 11)及粒細胞巨噬細胞集落刺激因子(CSF2),下調最為明顯的為腫瘤壞死因子超傢族成員5(CD40)、白介素10(IL-10)及B細胞κ輕肽基因增彊子覈因子抑製因子ε(NFκBIE).結論 在檢測的銀屑病患者皮損組織中,NF-κB信號通路相關的多種分子可能處于被激活狀態或抑製狀態.
목적 검측유오이족은설병환자피손조직여핵인자κB(NF-κB)상관적84개신호분자적표체,초보탐토표체명현이상적신호분자.방법 수집8례신강유오이족은설병환자피손조직,동시수집환자적정상피부조직작위대조,제취조직총RNA,역전록생성cDNA후,취합매련반응Array법(PCR-Array)검측NF-κB상관적84개신호분자표체수평,이2배위상조혹자하조적검험표준.결과 84충신호분자중,22충신호분자표체상조,7충하조.기중,상조최위명현적분자위광동태매모집결구역(CARD 11)급립세포거서세포집락자격인자(CSF2),하조최위명현적위종류배사인자초가족성원5(CD40)、백개소10(IL-10)급B세포κ경태기인증강자핵인자억제인자ε(NFκBIE).결론 재검측적은설병환자피손조직중,NF-κB신호통로상관적다충분자가능처우피격활상태혹억제상태.
Objective To detect the expression of 84 signaling molecules associated with nuclear factor-κB in lesions of Uygur patients with psoriasis.Methods Skin specimens were obtained from the lesional and paralesional skin of eight Uygur patients with psoriasis.Total RNA was extracted from the skin specimens and reversely transcribed into cDNA.PCR-array analysis was carried out to quantify the expressions of 84 signaling molecules related to nuclear factor-κB.Genes with a fold-change ＞ or =2 were defined as differentially expressed.Results Among the 84 tested genes,22 showed upregulated expression,7 downregulated expression,and the remaining 54 genes showed no significant changes in psoriatic lesions compared with the normal skin.The strongest upregulation was observed in the gene expressions of Caspase recruitment domain family 11 (CARD11) and granulocyte-macrophage colony-stimulating factor 2 (CSF2),and the most significant downregulation in the gene expression of interleukin 10 (IL-10),tumor necrosis factor superfamily member 5 (CD40) and nuclear factor of kappa light polypeptide gene enhancer in B-cell inhibitor,epsilon (NFκBIE).Conclusion Multiple molecules involved in the NF-κB signaling pathway might be activated or inhibited in lesions of patients with psoriasis.
