中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
12期
1864-1869
,共6页
古丽巴努?依马木%徐国强%迪丽努尔?阿吉%古则丽阿依?阿不都卡德尔%刘佳怡%徐彬%李琦
古麗巴努?依馬木%徐國彊%迪麗努爾?阿吉%古則麗阿依?阿不都卡德爾%劉佳怡%徐彬%李琦
고려파노?의마목%서국강%적려노이?아길%고칙려아의?아불도잡덕이%류가이%서빈%리기
生物材料%材料生物相容性%硅烷偶联剂%硅烷化%钛片%骨髓间充质干细胞%细胞相容性%国家自然科学基金
生物材料%材料生物相容性%硅烷偶聯劑%硅烷化%鈦片%骨髓間充質榦細胞%細胞相容性%國傢自然科學基金
생물재료%재료생물상용성%규완우련제%규완화%태편%골수간충질간세포%세포상용성%국가자연과학기금
biocompatible materials%silanes%titanium%stem cells
背景:目前国内将硅烷偶联用于金属表面预处理的报道较少。
<br> 目的:对NaOH碱处理的钛片行硅烷化改性,观察硅烷膜的表面形貌与结构特征及细胞相容性。
<br> 方法:对制得的纯钛试件行NaOH碱处理,然后分别以8%,15%,33%浓度的KH-550硅烷偶联剂进行改性处理,以纯钛片和碱处理的钛片为对照组,采用扫描电镜观察改性处理钛片表面微观形貌,采用能谱仪分析改性处理钛片表面的成分。将纯钛片、碱处理的钛片及不同浓度 KH-550硅烷偶联剂改性处理的钛片分别与犬骨髓间充质干细胞共培养,观察材料表面细胞的形态及黏附。
<br> 结果与结论:硅烷膜由许多呈脑浆状的小片构成,排列紧密,主要由C、N、O、Si等元素组成。当硅烷溶液浓度为8%时,钛表面难以形成较完整的硅烷膜;当硅烷溶液浓度为15%时,钛表面形成的硅烷膜表面较8%浓度组钛表面相对较完整,但可仍见有较多裂纹,难以形成致密的硅烷膜;当硅烷溶液浓度为33%时,纯钛表面能形成致密的硅烷膜。犬骨髓间充质干细胞在33%浓度硅烷膜处理后钛基体上的黏附情况明显优于纯钛、碱处理钛片及8%,15%浓度硅烷膜处理后的钛片。说明33%硅烷化改性纯钛表面硅烷膜较完整,并且具有良好的生物相容性,可促进骨髓间质干细胞在活性层表面的黏附。
揹景:目前國內將硅烷偶聯用于金屬錶麵預處理的報道較少。
<br> 目的:對NaOH堿處理的鈦片行硅烷化改性,觀察硅烷膜的錶麵形貌與結構特徵及細胞相容性。
<br> 方法:對製得的純鈦試件行NaOH堿處理,然後分彆以8%,15%,33%濃度的KH-550硅烷偶聯劑進行改性處理,以純鈦片和堿處理的鈦片為對照組,採用掃描電鏡觀察改性處理鈦片錶麵微觀形貌,採用能譜儀分析改性處理鈦片錶麵的成分。將純鈦片、堿處理的鈦片及不同濃度 KH-550硅烷偶聯劑改性處理的鈦片分彆與犬骨髓間充質榦細胞共培養,觀察材料錶麵細胞的形態及黏附。
<br> 結果與結論:硅烷膜由許多呈腦漿狀的小片構成,排列緊密,主要由C、N、O、Si等元素組成。噹硅烷溶液濃度為8%時,鈦錶麵難以形成較完整的硅烷膜;噹硅烷溶液濃度為15%時,鈦錶麵形成的硅烷膜錶麵較8%濃度組鈦錶麵相對較完整,但可仍見有較多裂紋,難以形成緻密的硅烷膜;噹硅烷溶液濃度為33%時,純鈦錶麵能形成緻密的硅烷膜。犬骨髓間充質榦細胞在33%濃度硅烷膜處理後鈦基體上的黏附情況明顯優于純鈦、堿處理鈦片及8%,15%濃度硅烷膜處理後的鈦片。說明33%硅烷化改性純鈦錶麵硅烷膜較完整,併且具有良好的生物相容性,可促進骨髓間質榦細胞在活性層錶麵的黏附。
배경:목전국내장규완우련용우금속표면예처리적보도교소。
<br> 목적:대NaOH감처리적태편행규완화개성,관찰규완막적표면형모여결구특정급세포상용성。
<br> 방법:대제득적순태시건행NaOH감처리,연후분별이8%,15%,33%농도적KH-550규완우련제진행개성처리,이순태편화감처리적태편위대조조,채용소묘전경관찰개성처리태편표면미관형모,채용능보의분석개성처리태편표면적성분。장순태편、감처리적태편급불동농도 KH-550규완우련제개성처리적태편분별여견골수간충질간세포공배양,관찰재료표면세포적형태급점부。
<br> 결과여결론:규완막유허다정뇌장상적소편구성,배렬긴밀,주요유C、N、O、Si등원소조성。당규완용액농도위8%시,태표면난이형성교완정적규완막;당규완용액농도위15%시,태표면형성적규완막표면교8%농도조태표면상대교완정,단가잉견유교다렬문,난이형성치밀적규완막;당규완용액농도위33%시,순태표면능형성치밀적규완막。견골수간충질간세포재33%농도규완막처리후태기체상적점부정황명현우우순태、감처리태편급8%,15%농도규완막처리후적태편。설명33%규완화개성순태표면규완막교완정,병차구유량호적생물상용성,가촉진골수간질간세포재활성층표면적점부。
BACKGROUND:Nowadays, silane coupling for metal surface pretreatment has not been widely applied yet. OBJECTIVE:To observe the surface appearance, structure and biocompatibility of NaOH pre-treated titanium sheet coated with silane films.
<br> METHODS:The NaOH pre-treated titanium sheet was silanized with the silane coupler (KH-550) at different concentrations (8%, 15%and 33%). The test specimens manufactured with different concentrations of silane treatment solutions were analyzed with scanning electron microscope and energy dispersive spectroscopy analyzer, in comparison with pure titanium and alkali-pretreated titanium sheets as controls. After silanization, bone marrow mesenchymal stem cel s from dogs were co-cultured with the test specimens to observe the cel morphology and adhesion on the surface of test specimens.
<br> RESULTS AND CONCLUSION:The silane film consisted of brain shaped sheets which arranged closely. The composition of the silane film was C, N, O and Si. The test specimen coated with the 33%silane solution had the ful silane film, while the silane film was imperfect at a concentration of 8%. The cel adhesion was better on the surface of test specimens treated with 33%silane solution than the other specimens. These findings indicate that the titanium sheet modified with 33%silane solution possesses a good biocompatibility, thereby improving the cel adhesion, spreading, proliferation and differentiation.