CAJ | 학술논문

背景：目前听骨链重建仍是治疗传导性聋的重要方法，多种生物材料被应用于听骨链重建，但都忽略了听骨植入后的血液供应，更未形成真正意义的骨组织。 　　目的：观察经血管内皮生长因子混合Ⅰ型胶原修饰β-磷酸三钙多孔支架植入豚鼠听泡内的血管化效果。 　　方法：取豚鼠60只制作听泡内壁黏膜缺损创面，随机分组，实验组听泡内植入经血管内皮生长因子混合Ⅰ型胶原修饰的β-磷酸三钙多孔支架，对照组植入Ⅰ型胶原修饰的β-磷酸三钙多孔支架，空白对照组植入β-磷酸三钙多孔支架，植入后1，2，3，4周扫描电镜观察支架表面情况，苏木精-伊红及免疫组织化学染色观察支架内血管生成情况，甲苯胺蓝染色观察支架内新骨生成。 　　结果与结论：实验组植入1周后内皮细胞大量生长，血管管腔形成，3周时达血管生长高峰并可见微孔间交通支形成；另两组2周时可见血管管腔形成，但无微孔间交通支形成，且实验组各时间段血管计数均高于对照组和空白对照组(P<0.05)。3组支架表面及微孔内细胞黏附生长，形态基本一致。植入4周时实验组支架内成骨较其他两组明显。表明经血管内皮生长因子混合Ⅰ型胶原修饰的β-磷酸三钙多孔支架在豚鼠中耳环境中能够有效实现血管化，促进支架内新骨形成。
배경：목전은골련중건잉시치료전도성롱적중요방법，다충생물재료피응용우은골련중건，단도홀략료은골식입후적혈액공응，경미형성진정의의적골조직。 　　목적：관찰경혈관내피생장인자혼합Ⅰ형효원수식β-린산삼개다공지가식입돈서은포내적혈관화효과。 　　방법：취돈서60지제작은포내벽점막결손창면，수궤분조，실험조은포내식입경혈관내피생장인자혼합Ⅰ형효원수식적β-린산삼개다공지가，대조조식입Ⅰ형효원수식적β-린산삼개다공지가，공백대조조식입β-린산삼개다공지가，식입후1，2，3，4주소묘전경관찰지가표면정황，소목정-이홍급면역조직화학염색관찰지가내혈관생성정황，갑분알람염색관찰지가내신골생성。 　　결과여결론：실험조식입1주후내피세포대량생장，혈관관강형성，3주시체혈관생장고봉병가견미공간교통지형성；령량조2주시가견혈관관강형성，단무미공간교통지형성，차실험조각시간단혈관계수균고우대조조화공백대조조(P<0.05)。3조지가표면급미공내세포점부생장，형태기본일치。식입4주시실험조지가내성골교기타량조명현。표명경혈관내피생장인자혼합Ⅰ형효원수식적β-린산삼개다공지가재돈서중이배경중능구유효실현혈관화，촉진지가내신골형성。
BACKGROUND:The auditory ossicle chain reconstruction is stil an important method to treat conductive deafness. Although a great variety of materials have been applied, the blood supply of otosteon after the implantation is ignored. Moreover, there is no real bone formed. OBJECTIVE:To observe the angiogenesis of vascular endothelial growth factor and col agen I modifiedβ-tricalcium phosphate porous scaffold which is implanted into the otocyst of guinea pig. METHODS:Total y 60 guinea pigs were randomly divided into experimental group (vascular endothelial growth factor and col agen I modifiedβ-tricalcium phosphate porous scaffold), col agen I control group (col agen I modifiedβ-tricalcium phosphate porous scaffold) and blank control group (β-tricalcium phosphate porous scaffold). The guinea pigs were executed under anesthesia at weeks 1, 2, 3, 4 respectively. The surface of scaffolds was observed by scanning electron microscopy. The angiogenesis of scaffolds were observed by hematoxylin-eosin staining and CD34 immunohistochemistry staining, and then the microvascular density was counted. The osteogenesis of the scaffolds was observed by toluidine blue staining. RESULTS AND CONCLUSION:Endothelial cel proliferation and lumen formation could be observed after 1 week in the experimental group, and the angiogenesis reach the peak after 3 weeks with traffic branches formedbetween micropores. In the other two groups, the lumen formed at 2 weeks but no traffic branches were visible. The sprouting of new blood vessels in the pores were observed more in the experimental group than the other two groups (P<0.05). The adherence and proliferation of cel s could be examined in the surface and pores of the scaffold by scanning electron microscope. After 4 weeks, the osteogenesis could be observed by toluidine blue staining, especial y in the experimental group. These findings suggest that the vascular endothelial growth factor and col agen I modifiedβ-tricalcium phosphate porous scaffold can realize an effective vascularization in the environment of guinea pigs’ middle ear. What’s more, the scaffold also can promote bone formation.