CAJ | 학술논문

背景：椎体成形、椎体后凸成形治疗骨质疏松性椎体压缩性骨折中存在骨水泥外溢、伤椎及临近椎体继发骨折等风险。 　　目的：验证高分子可降解网状微孔球囊应用于骨质疏松性椎体压缩骨折的可行性。 　　方法：以聚乳酸-已内酯共聚物为原材料，采用静电纺丝技术制备可降解网状微孔球囊，同时制备涂层球囊，扫描电镜观察其形貌；向球囊内分别注入水和骨水泥，观察外渗情况；测试球囊的断裂强度和断裂伸长率。采用细胞染色法和CCK-8法测试小鼠胚胎成骨细胞在两种球囊上的增殖情况。观察两种球囊在模拟体液、猪胰脂肪酶溶液和新鲜的人血清中的降解。将球囊置入猪椎体后测试球囊的爆破压。将钙盐骨水泥注入球囊后密封，固化后置于6 atm压力的超纯水中，定期检测钙离子浓度。 　　结果与结论：网状微孔球囊具有良好的纤维形态，粗细分布均匀，存在孔隙；涂层球囊表面无具体形态结构及孔隙存在。与涂层球囊相比，网状微孔球囊具有更好的力学性能和液体渗透性及爆破压力，可防止骨水泥渗漏，促进成骨细胞的黏附与增殖，降解更均匀、稳定，可较好保持钙离子浓度，更利于新骨生长和骨折愈合。
배경：추체성형、추체후철성형치료골질소송성추체압축성골절중존재골수니외일、상추급림근추체계발골절등풍험。 　　목적：험증고분자가강해망상미공구낭응용우골질소송성추체압축골절적가행성。 　　방법：이취유산-이내지공취물위원재료，채용정전방사기술제비가강해망상미공구낭，동시제비도층구낭，소묘전경관찰기형모；향구낭내분별주입수화골수니，관찰외삼정황；측시구낭적단렬강도화단렬신장솔。채용세포염색법화CCK-8법측시소서배태성골세포재량충구낭상적증식정황。관찰량충구낭재모의체액、저이지방매용액화신선적인혈청중적강해。장구낭치입저추체후측시구낭적폭파압。장개염골수니주입구낭후밀봉，고화후치우6 atm압력적초순수중，정기검측개리자농도。 　　결과여결론：망상미공구낭구유량호적섬유형태，조세분포균균，존재공극；도층구낭표면무구체형태결구급공극존재。여도층구낭상비，망상미공구낭구유경호적역학성능화액체삼투성급폭파압력，가방지골수니삼루，촉진성골세포적점부여증식，강해경균균、은정，가교호보지개리자농도，경리우신골생장화골절유합。
BACKGROUND:Vertebroplasty and kyphoplasty for osteoporotic vertebral compression fractures can result in many complications, such as bone cement leakage and adjacent-level fractures. OBJECTIVE:To verify the possibility of biodegradable mesh-like microporous polymer bal oon for the treatment of osteoporotic vertebral compression fractures. METHODS:Biodegradable mesh-like microporous P(DLLA-CL) bal oons were fabricated by electrospinning technique. Coated bal oons with the same specification was fabricated by coating P(DLLA-CL) onto the same mould. Morphology of the bal oons was observed by scanning electron microscopy. The bal oon leakage was observed by eyes after the injection of water or cement. The initial strength and stiffness were measured by a universal testing machine. The proliferation of MC3T3-E1 cel s on the bal oons was determined by laser confocal microscope and cel counting kit-8 assays. The biodegradation of bal oons in simulated body fluid, porcine pancreatic lipase, and fresh human serum was studied by residual weighing and scanning electron microscopy observation. Burst pressure of bal oons was measured after the bal oon was placed into a hole in the vertebral bone. For the in vitro calcium release tests, the bal oons were fil ed with calcium cement, tied, placed into 6atm ultrapure water, and then the calcium concentration was regularly determined. RESULTS AND CONCLUSION:Mesh-like microporous bal oons presented with good fiber morphology, thickness distribution, and the presence of pores;on the coated bal oon surface, there was absence of specific morphology and porosity. Compared with the coated bal oon, the mesh-like microporous bal oon showed better mechanical properties, liquid permeability and burst pressure, to prevent leakage of bone cement and promote osteoblast adhesion and proliferation. In addition, the degradation of the mesh-like microporous bal oons was more uniform and stable than the coated bal oons, which may increase the calcium concentration in the injured vertebrae and wil be beneficial to the new bone growth and fracture healing.