中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2014年
10期
1009-1013,1023
,共6页
黄经纬%郑予桐%李家璜%华子春
黃經緯%鄭予桐%李傢璜%華子春
황경위%정여동%리가황%화자춘
硫氧还蛋白谷胱甘肽还原酶%日本血吸虫%同源建模%功能分析
硫氧還蛋白穀胱甘肽還原酶%日本血吸蟲%同源建模%功能分析
류양환단백곡광감태환원매%일본혈흡충%동원건모%공능분석
thioredoxin glutathione reductase%Schistosoma j aponicum%homologous modeling%function analysis
目的:对日本血吸虫硫氧还蛋白谷胱甘肽还原酶(TGR)进行结构及功能分析。方法基于结构序列比较利用Swiss-Pdbviewer构建了日本血吸虫的TGR同源结构模型,并对模型进行结构评估;分析日本血吸虫TGR与底物结合时可能的位点,比较这些位点在不同来源TGR中的异同。结果日本血吸虫 TGR结构在 PROCHECK评估中被证实可靠;位点比较分析表明NADPH、GDS结合区是保守的位点;GSH结合区存在特异性。结论作用于GDS、NADPH结合区的其它来源的TGR抑制剂可能对日本血吸虫TGR也有作用;GSH结合区是设计寄生虫TGR特异性抑制剂的潜在靶点之一;TGR的C末端对电子传递起着重要作用并可能参与底物的结合,因而阻断日本血吸虫TGR的C末端摆动的抑制剂将可能有效地抑制日本血吸虫TGR活性。
目的:對日本血吸蟲硫氧還蛋白穀胱甘肽還原酶(TGR)進行結構及功能分析。方法基于結構序列比較利用Swiss-Pdbviewer構建瞭日本血吸蟲的TGR同源結構模型,併對模型進行結構評估;分析日本血吸蟲TGR與底物結閤時可能的位點,比較這些位點在不同來源TGR中的異同。結果日本血吸蟲 TGR結構在 PROCHECK評估中被證實可靠;位點比較分析錶明NADPH、GDS結閤區是保守的位點;GSH結閤區存在特異性。結論作用于GDS、NADPH結閤區的其它來源的TGR抑製劑可能對日本血吸蟲TGR也有作用;GSH結閤區是設計寄生蟲TGR特異性抑製劑的潛在靶點之一;TGR的C末耑對電子傳遞起著重要作用併可能參與底物的結閤,因而阻斷日本血吸蟲TGR的C末耑襬動的抑製劑將可能有效地抑製日本血吸蟲TGR活性。
목적:대일본혈흡충류양환단백곡광감태환원매(TGR)진행결구급공능분석。방법기우결구서렬비교이용Swiss-Pdbviewer구건료일본혈흡충적TGR동원결구모형,병대모형진행결구평고;분석일본혈흡충TGR여저물결합시가능적위점,비교저사위점재불동래원TGR중적이동。결과일본혈흡충 TGR결구재 PROCHECK평고중피증실가고;위점비교분석표명NADPH、GDS결합구시보수적위점;GSH결합구존재특이성。결론작용우GDS、NADPH결합구적기타래원적TGR억제제가능대일본혈흡충TGR야유작용;GSH결합구시설계기생충TGR특이성억제제적잠재파점지일;TGR적C말단대전자전체기착중요작용병가능삼여저물적결합,인이조단일본혈흡충TGR적C말단파동적억제제장가능유효지억제일본혈흡충TGR활성。
To explore the structure and function of thioredoxin glutathione reductase (TGR) from Schistosoma j aponi-cum ,the homologous model of TGR in Schistosoma j aponicum was constructed by Swiss-Pdbviewer based on sequence and structure alignment .The potential substrates binding sites of TGR were analyzed and these sites of various TGRs were also as-sessed .Our results showed that the homologous model of Schistosoma japonicum TGR based on Schistosoma mansoni TGR structure was proved to be reasonable by PROCHECK program .Analysis of binding sites showed that NADPH and GDS bind-ing sites were conservative sites and GSH binding site was a specific site for parasite .Our data suggested that inhibitors which work in NADPH and GDS binding sites of other various TGRs may also interact with TGR form Schistosoma j aponicum .GSH binding region might be one of the potential targets for design of specific inhibitors of parasite TGRs .In addition ,C-terminal of TGR plays an important role in electron transfer and may participate in the binding of the substrate .Thus compound inhibiting swing of C-terminal could effectively restrain Schistosoma j aponicum TGR activity .