CAJ | 학술논문

目的：为了解郑州地区牛携带大肠杆菌O157∶ H7的情况。方法应用本实验室已建立的大肠杆菌 O157∶ H7多重PCR方法对其进行了检测，并对临床分离的动物源大肠杆菌O157∶ H7的生物学特性及携带的毒力基因情况进行了分析。结果所采集的样品中共分离鉴定出2株大肠杆菌 O157∶ H7，分别命名为L1和L2，其检出率为1．4％；临床分离菌株的生化试验结果均符合大肠杆菌的常规生化特性；L1和L2菌株的生长曲线一致，均比标准株的迟缓期短，较早进入对数生长期；L1菌株在48 h可形成成熟完整的生物被膜，L2菌株在36 h形成成熟完整的生物被膜；L1和L2菌株均携带有hlyA和eaeA毒力基因，同时L1还携带Stx2毒力基因。结论该结果为大肠杆菌O157∶H7流行病学调查提供了相关数据，对郑州地区大肠杆菌O157∶H7有效的监测奠定了基础。
목적：위료해정주지구우휴대대장간균O157∶ H7적정황。방법응용본실험실이건립적대장간균 O157∶ H7다중PCR방법대기진행료검측，병대림상분리적동물원대장간균O157∶ H7적생물학특성급휴대적독력기인정황진행료분석。결과소채집적양품중공분리감정출2주대장간균 O157∶ H7，분별명명위L1화L2，기검출솔위1．4％；림상분리균주적생화시험결과균부합대장간균적상규생화특성；L1화L2균주적생장곡선일치，균비표준주적지완기단，교조진입대수생장기；L1균주재48 h가형성성숙완정적생물피막，L2균주재36 h형성성숙완정적생물피막；L1화L2균주균휴대유hlyA화eaeA독력기인，동시L1환휴대Stx2독력기인。결론해결과위대장간균O157∶H7류행병학조사제공료상관수거，대정주지구대장간균O157∶H7유효적감측전정료기출。
To understand the Escherichia coli (E .coli) O157∶ H7 isolated from cow in Zhengzhou ,Henan Province ,a total of 146 samples of cow fecal and milk were collected in the different farms ,and E .coli O157∶ H7 was detected with mul-tiplex polymerase chain reaction (PCR) in our laboratory .Then the biochemical characteristics ,growth dynamic ,the biofilm formation ,and the toxin genes of the E .coli O157∶ H7 isolates were analyzed .The results showed that 2 strains of E .coli O157∶H7 were found ,with the detection rate of 1 .4% ,and the isolates were named as L1 and L2 in current study ,respec-tively .The E .coli O157∶H7 clinical isolates had the same biochemical characteristics with that of the typical E .coli .The L1 and L2 isolates presented similar growth curve ,which entered into the log phase earlier than that of the standard strain .L1 strain formed thick ,confluent ,complete biofilm after 48 hours post-inoculation ,and the biofilm of L2 strain was formed com-pletely in 36 hours .The two E .coli O157∶ H7 isolates were positive with eaeA and hlyA genes ,and the L1 strain also carried the Stx2 virulence gene .Our results reinforce the epidemiological data of E .coli O157∶H7 ,and underscore the need for more effective surveillance of animal-derived E .coli O157∶H7 isolates in Zhengzhou City ,China .