CAJ | 학술논문

目的：探讨BCG初次免疫，IL-12联合结核分枝杆菌Ag85A DNA疫苗加强免疫对小鼠的免疫效果。方法实验小鼠随机分为7组，即 PBS阴性对照组、BCG组、pcAg85A组、BCG初免pcAg85A加强免疫组、BCG初免pcAg85A联合IL-12加强免疫组、BCG初免IL-12加强免疫组、以及BCG初免pcDNA3．1加强免疫组。按BCG初免，细胞因子IL-12联合结核分枝杆菌Ag85A DNA加强的免疫程序进行免疫实验，在末次免疫后的4、6、8周通过检测小鼠血清总IgG抗体、特异性淋巴细胞增殖，细胞因子的水平，观测对小鼠的免疫效果。结果采用BCG初免，细胞因子IL-12联合结核分枝杆菌Ag85A DNA疫苗加强的免疫策略组的小鼠与其它免疫方式组相比，IgG明显升高（P＜0．05）、特异性淋巴细胞明显增殖，加强免疫后IFN-γ水平、IL-2水平、IL-4水平BCG/Ag85A＋ IL-12组在3个时间段分别为128．2±20．4、190．2±16．51、244．2±39．14；146．2±17．29、271．6±16．36、419．3±28．12；68．6±6．62、96．6±5．5、117．4±10．71均高于其它各组（ P＜0．05）。结论采用BCG初免，细胞因子IL-12联合结核分枝杆菌Ag85A DNA疫苗加强的免疫能明显增强机体体液和细胞免疫，为进一步在动物体内进行保护性效应试验的研究提供了依据。
목적：탐토BCG초차면역，IL-12연합결핵분지간균Ag85A DNA역묘가강면역대소서적면역효과。방법실험소서수궤분위7조，즉 PBS음성대조조、BCG조、pcAg85A조、BCG초면pcAg85A가강면역조、BCG초면pcAg85A연합IL-12가강면역조、BCG초면IL-12가강면역조、이급BCG초면pcDNA3．1가강면역조。안BCG초면，세포인자IL-12연합결핵분지간균Ag85A DNA가강적면역정서진행면역실험，재말차면역후적4、6、8주통과검측소서혈청총IgG항체、특이성림파세포증식，세포인자적수평，관측대소서적면역효과。결과채용BCG초면，세포인자IL-12연합결핵분지간균Ag85A DNA역묘가강적면역책략조적소서여기타면역방식조상비，IgG명현승고（P＜0．05）、특이성림파세포명현증식，가강면역후IFN-γ수평、IL-2수평、IL-4수평BCG/Ag85A＋ IL-12조재3개시간단분별위128．2±20．4、190．2±16．51、244．2±39．14；146．2±17．29、271．6±16．36、419．3±28．12；68．6±6．62、96．6±5．5、117．4±10．71균고우기타각조（ P＜0．05）。결론채용BCG초면，세포인자IL-12연합결핵분지간균Ag85A DNA역묘가강적면역능명현증강궤체체액화세포면역，위진일보재동물체내진행보호성효응시험적연구제공료의거。
The aim of this study is to investigate the immune effects of BCG primary immunization and IL-12 combined with Mycobacterium tuberculosis Ag85A DNA vaccine booster immunization on mice .We randomly divided the mice into 7 groups ,namely PBS negative controls ,BCG controls ,pcAg85A controls ,BCG primary immunization combined with Ag85A booster immunization controls ,BCG primary immunization combined with Ag85A and IL-12 booster immunization controls , BCG primary immunization combined with IL-12 booster immunization controls ,and BCG primary immunization combined with pcDNA3 .1 booster immunization controls .Implementing the immune in procedure of BCG primary immunization and cytokine IL-12 booster immunization combined with mycobacterium tuberculosis Ag85A DNA ,we observed the immune effect on mice by detecting the mice serum total IgG , specific lymphocyte proliferation and cytokine levels in 4 ,6 ,8 weeks after the last immunization .Comparing the mice immunized in the strategy of BCG primary immunization and cytokine IL-12 combined with Mycobacterium tuberculosis Ag85A DNA vaccine strengthening immunization to the mice in other groups by other immune ways ,we found that ,in BCG/Ag85A+ IL-12 groups ,IgG in-creased significantly (P< 0 .05) ,specific lymphocyte prolifera-ted significantly ,and after strengthening immunization IFN-γlevels ,IL-2 levels and IL-4 levels in the three periods were 128 .2 ±20.4,190.2±16.51,244.2±39.14 ;146.2±17.29,271.6±16.36and16.36±28.12 ;68.6±6.62,96.6±5.5and5.5± 10 .71 ,respectively ,which were higher than those in other groups (P<0 .05) .It’s suggested that the immunization way of BCG primary immunization and cytokine IL-12 combined with Mycobacterium tuberculosis Ag85A DNA vaccine booster immu-nization could significantly enhance the humoral and cellular immunity of the bodies ,and provide the basis for further study on protective effect test in animals .