CAJ | 학술논문

目的：探讨IL-17在类风湿关节炎（ RA）发病中的作用及意义。方法：采用逆转录-聚合酶链反应（ RT-PCR）法检测66例RA患者（活动期34例、缓解期32例）和44例健康对照组外周血单个核细胞（ PBMC）中IL-17 mRNA 的表达水平，采用酶联免疫吸附试验（ ELISA）检测各组血清中IL-17的浓度，用免疫比浊法检测各组血清RF的浓度，分析血清IL-17与RF的相关性。结果：RA患者PBMC中IL-17 mRNA的表达显著高于健康对照组（ P＜0.05），但活动期与缓解期无明显差异（ P＞0.05）；RA活动期患者血清IL-17水平显著高于RA缓解期患者和健康对照组（ P＜0.05）；且RA患者血清IL-17水平与RF呈正相关。结论：IL-17和RF在RA的发病和病理过程中起重要作用，IL-17可能与RA活动性有关。
목적：탐토IL-17재류풍습관절염（ RA）발병중적작용급의의。방법：채용역전록-취합매련반응（ RT-PCR）법검측66례RA환자（활동기34례、완해기32례）화44례건강대조조외주혈단개핵세포（ PBMC）중IL-17 mRNA 적표체수평，채용매련면역흡부시험（ ELISA）검측각조혈청중IL-17적농도，용면역비탁법검측각조혈청RF적농도，분석혈청IL-17여RF적상관성。결과：RA환자PBMC중IL-17 mRNA적표체현저고우건강대조조（ P＜0.05），단활동기여완해기무명현차이（ P＞0.05）；RA활동기환자혈청IL-17수평현저고우RA완해기환자화건강대조조（ P＜0.05）；차RA환자혈청IL-17수평여RF정정상관。결론：IL-17화RF재RA적발병화병리과정중기중요작용，IL-17가능여RA활동성유관。
Objective:To explore the role and significance of IL-17 on the pathogenesis of rheumatoid arthritis ( RA).Methods:The levels of IL-17 mRNA in peripheral blood mononuclear cell ( PBMC) of 66 RA patients (32 active,34 inactive) and 44 healthy controls were detected by reverse transcription-polymerase chain reaction ( RT-PCR ).The levels of serum IL-17 were detected by enzyme-linked immunosorbent assay ( ELISA).The levels of RF were detected by immunoturbidimetric assay.The correlations of serum IL-17 and RF were analyzed.Results: In RA patients the levels of IL-17 mRNA in PBMC were significantly higher than those in healthy controls ( P<0.05 ) , but there was no difference between the active and inactive RA ( P>0.05 ).The serum IL-17 levels in active RA patients were significantly higher than those in inactive RA patients and healthy controls ( P<0.05 ).The levels of serum IL-17 were positively correlated with RF in RA patients.Conclusion: IL-17 and RF play an important role in the pathogenesis and pathology process of RA ,and IL-17 may be associated with RA activity.