CAJ | 학술논문

目的：定量研究白术水提物对酵母朊病毒[ PS I＋]的治愈作用。方法首先用含白术水提物的培养基培养酵母朊病毒[ PS I＋]阳性菌株，初步评价白术的治愈作用。然后在细胞水平借助影印培养法和蛋白水平上使用半变性琼脂糖凝胶电泳结合蛋白免疫技术进一步验证白术水提物对酵母朊病毒[ PS I＋]的治愈作用。结果白术水提物作用酵母朊病毒[PSI＋]5d后的治愈率为6％。结论白术水提物对酵母朊病毒[PSI＋]有一定的治愈作用。
목적：정량연구백술수제물대효모원병독[ PS I＋]적치유작용。방법수선용함백술수제물적배양기배양효모원병독[ PS I＋]양성균주，초보평개백술적치유작용。연후재세포수평차조영인배양법화단백수평상사용반변성경지당응효전영결합단백면역기술진일보험증백술수제물대효모원병독[ PS I＋]적치유작용。결과백술수제물작용효모원병독[PSI＋]5d후적치유솔위6％。결론백술수제물대효모원병독[PSI＋]유일정적치유작용。
Investigation of the effects of rhizome atractylodis macrocephalae on yeast prion [PSI+ ]was conducted in this study .Liquid culture containing rhizome atractylodis macrocephalae aqueous extracts was applied to evaluate its therapeutic effects preliminarily .Then yeast replica plating together with Semi Denaturing Detergent Agarose Gel Electrophoresis com-bined with western blot were used to further confirm the results .It's showed that the cure rate of aqueous extracts of rhizome atractylodis macrocephalae on yease prion [PSI+ ]could reach 6% .