CAJ | 학술논문

目的：囊型包虫病是一种广泛流行且危害严重的人兽共患寄生虫病。本文旨在分析流行于青南地区细粒棘球绦虫系统发育学及基因多态性。方法本文利用线粒体 DNA细胞色素氧化酶亚单位Ⅰ（COX Ⅰ）分子对收集到的流行于青海省的59例包虫病样本进行测序。总计71条序列（其中12条来自GenBank）碱基通过Clustal X软件比对，构建贝叶斯进化树。结果流行于青海省包虫病样本大部分与细粒棘球绦虫G1型聚在一枝，还有三个样本与多房棘球绦虫（AB018440）聚在一枝。虽大部分棘球绦虫基因型属于G1型，但各自的基因型各不相同，具有丰富的多样性。结论流行于青海省细粒棘球绦虫系统发育学比我们想象复杂。
목적：낭형포충병시일충엄범류행차위해엄중적인수공환기생충병。본문지재분석류행우청남지구세립극구조충계통발육학급기인다태성。방법본문이용선립체 DNA세포색소양화매아단위Ⅰ（COX Ⅰ）분자대수집도적류행우청해성적59례포충병양본진행측서。총계71조서렬（기중12조래자GenBank）감기통과Clustal X연건비대，구건패협사진화수。결과류행우청해성포충병양본대부분여세립극구조충G1형취재일지，환유삼개양본여다방극구조충（AB018440）취재일지。수대부분극구조충기인형속우G1형，단각자적기인형각불상동，구유봉부적다양성。결론류행우청해성세립극구조충계통발육학비아문상상복잡。
Cystic echinococcosis is one of the most widespread and severe zoonotic helminthic diseases .To understand the phylogeny and genetic polymorphism Echinococcus granulosus (E .granulosus) prevailed in south region of Qinghai Prov-ince ,partial fragment of cytochrome oxidase Ⅰ (COX Ⅰ ) gene sequences were used to infer the phylogenetic relationship of 59 collected samples of E .granulosus in Qinghai Province .Total 72 sequences (13 sequences from GenBank) were aligned using CLUSTAL X ,and then ,Bayesian analyses were performed in Mrbayes-3 .1 .2 .The results revealed that Echinococcus spp .isolates did not form a monophyletic group .The most samples clustered with E . granulosus strain (G1) (AB297617) , but showed high genetic polymorphism .Another three samples clustered with E .multilocularis (AB018440) ,while they showed complex phylogenetic relationships among them ,further indicating that Echinococcus spp .isolates from Qinghai Prov-ince may has a more complex evolutionary history than expected .