CAJ | 학술논문

采用平板对峙法筛选到了马铃薯坏疽病的生防菌 ZA1，其对马铃薯坏疽病菌的抑制率为71.83%；通过形态特征和生理生化测定，结合16S rDNA和gyrB基因序列同源性分析，鉴定该菌为莫海威芽孢杆菌Bacillus mojavensis。抑菌谱测定表明，菌株ZA1对番茄早疫病菌Aalternaria solani、马铃薯褐腐病菌Stysanus stemonitis、马铃薯干腐病菌Fusarium oxysporum和马铃薯炭疽病菌Colletotrichum coccodes的抑制率分别达64.30%、41.05%、61.42%和74.92%。在贮藏库中进行10倍液喷雾，对马铃薯坏疽病的防效达64.31%。
채용평판대치법사선도료마령서배저병적생방균 ZA1，기대마령서배저병균적억제솔위71.83%；통과형태특정화생리생화측정，결합16S rDNA화gyrB기인서렬동원성분석，감정해균위막해위아포간균Bacillus mojavensis。억균보측정표명，균주ZA1대번가조역병균Aalternaria solani、마령서갈부병균Stysanus stemonitis、마령서간부병균Fusarium oxysporum화마령서탄저병균Colletotrichum coccodes적억제솔분별체64.30%、41.05%、61.42%화74.92%。재저장고중진행10배액분무，대마령서배저병적방효체64.31%。
The inhibition rate of bacterial ZA1 obtained by dual culture method against potato pathogen Phoma foveata was 71.83%. The strain ZA1 was identified as Bacillus mojavensis based on morphology, physiological and biochemical characters, combined with 16S rDNA and gyrB gene sequence analysis. Inhibition rate of strain ZA1 against Alternaria solani, Stysanus stemonitis, Fusarium oxysporum and Colletotrichum coccodes were 64.30%, 41.05%, 61.42%and 74.92%, respectively. The control efficacy of strain ZA1 against potato gangrene was 64.31%by spraying fermentation broth with diluting for ten times on potato tube in storage period.