CAJ | 학술논문

本文研究枯草芽孢杆菌产生的脂肽化合物表面活性素surfactin对烟草花叶病毒病的防治效果，并检测烟草防卫相关基因的表达情况以解析抗病机理。利用6 mol/LHCl沉淀枯草芽孢杆菌OKB105的去细胞培养液，通过甲醇抽提，Sephadex LH20层析柱获得脂肽纯化物。经MALDI-TOF-MS和血平板溶血试验检测表明，纯化物中仅含有表面活性素surfactin。将表面活性素配成0.01 g/L溶液，处理烟草并接种TMV。结果表明， surfactin对烟草花叶病毒病有显著的防治效果，病斑抑制率为70.9%。通过Real-time PCR检测烟草的防卫相关基因的表达情况结果发现，烟草SA信号通路的PR1、PR3和PR5，以及ET通路的ETR1表达量显著提高，说明surfactin诱导了烟草依赖于SA和ET信号通路的诱导系统抗性（induced systemic resistance, ISR）反应。
본문연구고초아포간균산생적지태화합물표면활성소surfactin대연초화협병독병적방치효과，병검측연초방위상관기인적표체정황이해석항병궤리。이용6 mol/LHCl침정고초아포간균OKB105적거세포배양액，통과갑순추제，Sephadex LH20층석주획득지태순화물。경MALDI-TOF-MS화혈평판용혈시험검측표명，순화물중부함유표면활성소surfactin。장표면활성소배성0.01 g/L용액，처리연초병접충TMV。결과표명， surfactin대연초화협병독병유현저적방치효과，병반억제솔위70.9%。통과Real-time PCR검측연초적방위상관기인적표체정황결과발현，연초SA신호통로적PR1、PR3화PR5，이급ET통로적ETR1표체량현저제고，설명surfactin유도료연초의뢰우SA화ET신호통로적유도계통항성（induced systemic resistance, ISR）반응。
In order to interpret resistance mechanism, controlling effect of surfactin against TMV on tobacco and the expression pattern of defense-related genes were investigated. Liquid culture of Bacillus subtilis OKB105 was precipitated by 6 mol/L HCl. The crude extract of lipopeptides was dissolved by methanol and separated by LH20 chromatographic column. The crude extract detected surfactin only by MALDI-TOF-MS and hemolytic activity analysis. Tobacco was infected with TMV after pretreated by 0.01 g/L surfactin, the result showed that surfactin could control TMV efficiently, the controlling effect reached to 70.9%. The expressions of SA-pathway genes PR1, PR3, PR5, and ET-pathway gene ETR1 were obviously increased by real-time PCR test. The results demonstrated that surfactin could elicite ISR (induced systemic resistance) reaction which depend on SA-pathway and ET-pathway.