CAJ | 학술논문

目的：观察烟草浸提液（ST）对人牙周膜细胞（hPDLCs）增殖、热休克蛋白70（HSP70）表达的影响。方法：常规培养hPDLCs，第5～8代细胞用于实验。对照组为常规培养液，实验组为含不同浓度的 ST 培养液（0．016～50 g/L ST），作用24 h后 MTT 法检测细胞增殖、免疫组化和 Western Blot 法检测细胞 HSP70的表达。结果：ST 浓度为0．4～50 g/L 时抑制 hPDLCs增殖，增强细胞质及胞核内 HSP70的表达；ST 浓度增加，其作用更明显。结论：ST 可抑制 hPDLCs 增殖，促进 HSP70呈表达。
목적：관찰연초침제액（ST）대인아주막세포（hPDLCs）증식、열휴극단백70（HSP70）표체적영향。방법：상규배양hPDLCs，제5～8대세포용우실험。대조조위상규배양액，실험조위함불동농도적 ST 배양액（0．016～50 g/L ST），작용24 h후 MTT 법검측세포증식、면역조화화 Western Blot 법검측세포 HSP70적표체。결과：ST 농도위0．4～50 g/L 시억제 hPDLCs증식，증강세포질급포핵내 HSP70적표체；ST 농도증가，기작용경명현。결론：ST 가억제 hPDLCs 증식，촉진 HSP70정표체。
Objective:To study the effects of smokeless tobacco extract(ST)on the proliferation and the heat shock protein 70 (HSP70)expression of human periodontal ligament cells(hPDLCs).Methods:hPDLCs were cultured in vitro and identified by im-munohistochemistry(IHC).The cells were stimulated with ST at 0.01 6 -50 g/L respectively for 24 h,the proliferation was examine by MTT assay,HSP70 expression was detected by immunehistochemical staining and Western Blot.Results:ST inhibited the prolifer-ation and increased HSP70 expression in cytoplasm and nucleus at 0.4 -50 g/L dose dependantly.Conclusion:ST may inhibit the proliferation and increase HSP70 expression of hPDLCs in a dose depandant manner.