CAJ | 학술논문

目的：探讨环境蔗糖对寡发酵链球菌（So）抑制变形链球菌（Sm）作用及对 So 产过氧化氢能力的影响。方法：用平板培养法观察不同蔗糖环境下 So 与 Sm 之间的相互作用；用4-氨基安替吡啉-辣根过氧化物酶法测定 So 过氧化氢的初始产生速率和产量。结果：500 mmol/L 蔗糖环境下未见 So 对 Sm 的抑制作用，其它浓度蔗糖环境下 So 对 Sm 的抑制作用为：50 mmol/L ＞0 mmol/L 和1 mmol/L（P ＜0．05）；不同蔗糖环境下 So 过氧化氢初始产生速率为：50 mmol/L ＞0 mmol/L 和1 mmol/L＞500 mmol/L（P ＜0．05）；过氧化氢总产量为：0 mmol/L 和1 mmol/L ＞50 mmol/L ＞500 mmol/L（P ＜0．05）。当 So 先接种时对Sm 的抑制能力优于同时接种。结论：蔗糖浓度影响 So 对 Sm 的抑制能力及 So 产过氧化氢的能力。
목적：탐토배경자당대과발효련구균（So）억제변형련구균（Sm）작용급대 So 산과양화경능력적영향。방법：용평판배양법관찰불동자당배경하 So 여 Sm 지간적상호작용；용4-안기안체필람-랄근과양화물매법측정 So 과양화경적초시산생속솔화산량。결과：500 mmol/L 자당배경하미견 So 대 Sm 적억제작용，기타농도자당배경하 So 대 Sm 적억제작용위：50 mmol/L ＞0 mmol/L 화1 mmol/L（P ＜0．05）；불동자당배경하 So 과양화경초시산생속솔위：50 mmol/L ＞0 mmol/L 화1 mmol/L＞500 mmol/L（P ＜0．05）；과양화경총산량위：0 mmol/L 화1 mmol/L ＞50 mmol/L ＞500 mmol/L（P ＜0．05）。당 So 선접충시대Sm 적억제능력우우동시접충。결론：자당농도영향 So 대 Sm 적억제능력급 So 산과양화경적능력。
Objective:To investigate the inhibition of Streptococcus oligofermentans(So)on Streptococcus mutans(Sm)and the hydro-gen peroxide(H2 O2 )producibility by So under different sucrose concentration environment.Methods:The inhibition of So on Sm was observed by plating method under different sucrose concentration environment.The initial synthesis rates and production of H2 O2 by So were determined by 4-aminoantipyine-horseradish peroxidase method.Results:Under 500 mmol/L of H2 O2 ,the inhibition of So on Sm was not observed.Under the other sucrose concentration environment,the inhibition of So on Sm was as following:50 mmol/L >0 mmol/L and 1 mmol/L(P <0.05);the initial synthesis rates of H2 O2 by So under different sucrose concentrations were as following:50 mmol/L >0 mmol/L and 1 mmol/L >500 mmol/L(P <0.05);the total production of H2 O2 by So:0 mmol/L and 1 mmol/L >50 mmol/L >500 mmol/L(P <0.05).When So was inoculated before Sm,the inhibition of So on Sm was stronger than that when the two species were inoculated at the same time.Conclusion:The capability of the inhibition of So on Sm and the production of H2 O2 by So are influenced by sucrose concentration.