CAJ | 학술논문

目的：比较3种不同钛合金表面形貌对成纤维细胞生物学行为的影响。方法：通过机械加工法（M组）、直流电解蚀刻法（DC 组）、交流电解蚀刻法（AC 组）成形3种不同钛合金表面形貌，制备标准大小试件，使用场发射扫描电子显微镜（FE-SEM）观察其表面微观形态，将 L929成纤维细胞共同培养于钛合金表面，吖啶橙染色法检测初期粘附性；MTT 法检测表面细胞的增殖情况。结果：DC 组钛表面可见直径5～20μm 微孔，AC 组微孔分布更均匀，M组表面可见微沟纹，无微孔。细胞在3组钛片表面粘附良好，AC 组优于 DC 组和 M组。DC 组和 AC 组细胞增殖性优于 M组，第3～5天 AC 组细胞增殖性优于 DC组。结论：通过交流电解蚀刻法形成的兼具微米-亚微米-纳米三级结构的钛合金表面具有良好的生物相容性，能够促进成纤维细胞的早期附着及增殖。
목적：비교3충불동태합금표면형모대성섬유세포생물학행위적영향。방법：통과궤계가공법（M조）、직류전해식각법（DC 조）、교류전해식각법（AC 조）성형3충불동태합금표면형모，제비표준대소시건，사용장발사소묘전자현미경（FE-SEM）관찰기표면미관형태，장 L929성섬유세포공동배양우태합금표면，아정등염색법검측초기점부성；MTT 법검측표면세포적증식정황。결과：DC 조태표면가견직경5～20μm 미공，AC 조미공분포경균균，M조표면가견미구문，무미공。세포재3조태편표면점부량호，AC 조우우 DC 조화 M조。DC 조화 AC 조세포증식성우우 M조，제3～5천 AC 조세포증식성우우 DC조。결론：통과교류전해식각법형성적겸구미미-아미미-납미삼급결구적태합금표면구유량호적생물상용성，능구촉진성섬유세포적조기부착급증식。
Objective:To compare the effect of three different titanium alloy surface topographies on the biological character fibro-blasts.Methods:Three different titanium alloy Ti6Al4V surface topographies were created by machining(M),direct current electro-chemical etching(DC)and alternating current electrochemical etching(AC)respectively,and standard sized samples were prepared. The surface topographies were observed field emission scanning electron microscopy(FE-SEM).Mouse L929 fibroblasts were cultured on different surfaces.The attachment of cells was examined by acridine orange staining and observed under fluorescent microscope.The proliferation of cells was measured using MTT assay.Results:Tubles with the diamete of 5 -20 μm were observed on the surface of the sample of group DC,and more evenly distributed tubles on that of group AC.Micro-sulci were observed and no microtuble was found on the sample surface of group M.L929 cells were attached on the surface of all the samples in the 3 groups.More adhered and better strached cells were observed on AC treated surface.The proliferation of fibroblasts on AC and DC surfaces was superior to that on M treated surface and at the 3 -5 day the proliferation of fibroblasts on the AC surface was higher than that on DC treated surface.Con-clusion:AC treated surface with the micro-submicro-nano hierarchical topography of titanium alloy has a superior biocompatibility and can promote the early attachment and proliferation of the fibroblasts.