CAJ | 학술논문

为了解黄皮[Clausena lansium (Lour.) Skeels]果皮中的抗菌活性成分，采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱等方法，从黄皮果皮的乙醇提取物中分离得到10个化合物，经波谱学分析鉴定为：lansine (1)、3-甲酰基咔唑(2)、3-甲酰基-6-甲氧基咔唑(3)、6-甲氧基咔唑-3-羧酸甲酯(4)、(6R,7E,9S)-9-羟基-4,7-巨豆二烯-3-酮(5)、7-羟基香豆素(6)、8-羟基呋喃香豆素(7)、辛黄皮酰胺(8)、对羟基肉桂酸甲酯(9)和胡萝卜苷(10)。其中化合物5和9为首次从黄皮属植物中分离得到，化合物8的碳谱数据是首次报道。用滤纸片琼脂扩散法测定化合物的抗菌活性，结果表明，化合物1、2、3、6、7和9对金黄色葡萄球菌(Staphylococcus aureus)有抑制作用。这有利于更好地开发利用黄皮这一药食两用的水果资源。
위료해황피[Clausena lansium (Lour.) Skeels]과피중적항균활성성분，채용규효주색보、Sephadex LH-20응효주색보등방법，종황피과피적을순제취물중분리득도10개화합물，경파보학분석감정위：lansine (1)、3-갑선기잡서(2)、3-갑선기-6-갑양기잡서(3)、6-갑양기잡서-3-최산갑지(4)、(6R,7E,9S)-9-간기-4,7-거두이희-3-동(5)、7-간기향두소(6)、8-간기부남향두소(7)、신황피선알(8)、대간기육계산갑지(9)화호라복감(10)。기중화합물5화9위수차종황피속식물중분리득도，화합물8적탄보수거시수차보도。용려지편경지확산법측정화합물적항균활성，결과표명，화합물1、2、3、6、7화9대금황색포도구균(Staphylococcus aureus)유억제작용。저유리우경호지개발이용황피저일약식량용적수과자원。
In order to understand the chemical constituents of Clausena lansium (Lour.) Skeels, ten compounds were isolated from 95%EtOH extract of peels of C. lansium by silica gel and sephadex LH-20 column chromatography. On the basis of spectral data, their structures were identified as lansine (1), 3-formyl carbazole (2), 3-formyl-6-methoxy carbazole (3), 6-methoxycarbazole-3-carboxylate (4), (6R,7E,9S)-9-hydroxy-4,7-megastigmadien-3-one (5), umbelliferone (6), 8-hydroxyfurocoumarin (7), zeta-clausenamide (8), methyl p-hydroxycinnamate (9), and daucosterol (10). Compounds 5 and 9 were isolated from the genus Clausena for the ifrst time, and the 13C NMR spectral data of compound 8 was also reported for the ifrst time. Compounds 1, 2, 3, 6, 7 and 9 showed antibacterial activity on Staphylococcus aureus in a bioactive assay by using paper disk diffusion method. These would be helpful for exploitation of Clausena lansium.