CAJ | 학술논문

为探讨宁夏枸杞(Lycium barbarum)苯丙氨酸解氨酶基因(LbPAL)的表达特征，采用PCR法克隆了宁夏枸杞LbPAL基因的cDNA，并用实时定量PCR法分析了其表达特征。结果表明：宁夏枸杞的LbPAL基因的全长cDNA为2321 bp，包含2163 bp、编码720个氨基酸的开放阅读框；LbPAL与茄科其他物种的PAL氨基酸序列及三维结构具有较高保守性；与茄科物种的PAL聚类在同一个分支中。LbPAL在叶、花瓣、S1期果实的表达量较高，而在根及S2~S5期果实的表达水平较低。在NaCl胁迫处理下，LbPAL在根和茎中的表达量均有下调的趋势；而在叶片中，LbPAL表达量先急剧增加而后急剧下降并趋于稳定。这为解析宁夏枸杞中类黄酮化合物的生物合成调节及生理功能提供了参考。
위탐토저하구기(Lycium barbarum)분병안산해안매기인(LbPAL)적표체특정，채용PCR법극륭료저하구기LbPAL기인적cDNA，병용실시정량PCR법분석료기표체특정。결과표명：저하구기적LbPAL기인적전장cDNA위2321 bp，포함2163 bp、편마720개안기산적개방열독광；LbPAL여가과기타물충적PAL안기산서렬급삼유결구구유교고보수성；여가과물충적PAL취류재동일개분지중。LbPAL재협、화판、S1기과실적표체량교고，이재근급S2~S5기과실적표체수평교저。재NaCl협박처리하，LbPAL재근화경중적표체량균유하조적추세；이재협편중，LbPAL표체량선급극증가이후급극하강병추우은정。저위해석저하구기중류황동화합물적생물합성조절급생리공능제공료삼고。
In order to understand the expression of phenylalanine ammonia-lyase gene from Lycium barbarum L., named as LbPAL, the cDNA of LbPAL was cloned using PCR and its expression was characterized. The results showed that the whole length of LbPAL cDNA was 2321 bp containing an open reading frame (ORF) of 2163 bp, encoding 720 amino acids. Amino acid sequence analysis indicated that LbPAL had high identity of sequence and similarly tertiary structure to PALs in other Solanaceae plants. Phylogenetic analysis demonstrated that LbPAL was clustered in the same clade with PALs in other Solanaceae plants. Real-time PCR analysis showed that LbPAL was highly expressed in leaves, petals and fruits at S1 stage, while few LbPAL transcripts were detected in roots and fruits at stages from S2 to S5. Under salt stress, LbPAL expression was downregulated in roots and stems, while sharply enhanced at 0.5 h and decreased hereafter in leaves. These results lay a foundation for elucidating the biosynthesis and physiological regulation of lfavonoid in L. barbarum.