CAJ | 학술논문

采用外部换热中心移热式反应器合成了环形整体固定相基质，对基质进行化学改性制备了弱阴离子（DEAE）整体色谱柱，分别采用健合及吸附法将牛血清白蛋白（BSA）固载到基质上，制备适用于快速分离当归有效成分的径向亲和整体柱，并考察了整体色谱柱的分离性能。结果表明吸附牛血清白蛋白（BSA）色谱柱的分离性能优于健合蛋白色谱柱。
채용외부환열중심이열식반응기합성료배형정체고정상기질，대기질진행화학개성제비료약음리자（DEAE）정체색보주，분별채용건합급흡부법장우혈청백단백（BSA）고재도기질상，제비괄용우쾌속분리당귀유효성분적경향친화정체주，병고찰료정체색보주적분리성능。결과표명흡부우혈청백단백（BSA）색보주적분리성능우우건합단백색보주。
In this paper, a porous polymer monolith was prepared in a thermostated annular reactor. Weak anion ion-exchange radial flow monolithic columns were prepared by further surface modification of the stationary phase. Bovine serum albumin(BSA)was immobilized on the matrix, by means of adsorbed and bonded proteins respectively. The prepared radial flow affinity monolithic columns were then used to separate pharmaceutical constituents of Angelica. The separation efficiency of these monolithic columns was also investigated. It was shown that the affinity column prepared by adsorption of BSA achieved higher separation efficiency than the one with bonded BSA.