CAJ | 학술논문

【目的】探讨自体干细胞激活疗法对2型糖尿病（T2DM ）大鼠的降糖作用和对红细胞膜胰岛素受体数目及亲和力的影响。【方法】将实验大鼠分为健康对照组（NC组），糖尿病对照组（DC组），干细胞激活疗法治疗组（M I组），MI组给予rhG-CSF 3μg/（kg · d）隔日一次皮下注射，给予rhG-CSF d2开始每日行促肝细胞生长素3 mg/（kg · d）腹腔注射及烟酰胺100 mg/（kg · d）灌胃，连续28 d；NC组和DC组大鼠给予同体积生理盐水隔日一次皮下注射， d2开始每日给予同体积5％葡萄糖加中和量胰岛素腹腔注射、高压灭菌水灌胃，持续4周。实验结束后测定各组大鼠空腹血糖、空腹胰岛素、红细胞膜胰岛素受体数目和亲和力。【结果】造模后，与NC组对比，DC组、M I组大鼠空腹血糖、胰岛素抵抗指数（HOMA-IR）显著升高（ P＜0．01）；MI组较DC组血糖值下降33．95％，HOMA-IR显著降低（ P＜0．05）；DC组红细胞膜胰岛素受体数目较 NC组显著降低（ P＜0．05），MI组较DC组显著升高（ P＜0．05），三组间红细胞膜胰岛素受体亲和力无显著差异（ P＞0．05）。【结论】rh-CSF联合促肝细胞生长素和烟酰胺治疗可降低T2DM大鼠高血糖，并改善其胰岛素抵抗作用，其机制可能与提高细胞膜胰岛素受体数目相关。
【목적】탐토자체간세포격활요법대2형당뇨병（T2DM ）대서적강당작용화대홍세포막이도소수체수목급친화력적영향。【방법】장실험대서분위건강대조조（NC조），당뇨병대조조（DC조），간세포격활요법치료조（M I조），MI조급여rhG-CSF 3μg/（kg · d）격일일차피하주사，급여rhG-CSF d2개시매일행촉간세포생장소3 mg/（kg · d）복강주사급연선알100 mg/（kg · d）관위，련속28 d；NC조화DC조대서급여동체적생리염수격일일차피하주사， d2개시매일급여동체적5％포도당가중화량이도소복강주사、고압멸균수관위，지속4주。실험결속후측정각조대서공복혈당、공복이도소、홍세포막이도소수체수목화친화력。【결과】조모후，여NC조대비，DC조、M I조대서공복혈당、이도소저항지수（HOMA-IR）현저승고（ P＜0．01）；MI조교DC조혈당치하강33．95％，HOMA-IR현저강저（ P＜0．05）；DC조홍세포막이도소수체수목교 NC조현저강저（ P＜0．05），MI조교DC조현저승고（ P＜0．05），삼조간홍세포막이도소수체친화력무현저차이（ P＞0．05）。【결론】rh-CSF연합촉간세포생장소화연선알치료가강저T2DM대서고혈당，병개선기이도소저항작용，기궤제가능여제고세포막이도소수체수목상관。
[Objective]To explore the hypoglycemic effect of autologous stem cell activation therapy for type 2 diabetes mellitus(T2DM ) in rats and its impact on the number and affinity of insulin receptor on red cell mem-brane .[Methods] The experimental rats were divided into healthy control group (group NC) ,diabetes control group(group DC) and stem cell activation therapy group(group MI) .Group MI was given qod subcutaneous injec-tion of recombinant human granulocyte colony stimulating factor (rh-CSF) 3 μg/(kg · d) ,and then intraperitoneal injection of hepatocyte growth-promoting factor 3 mg/(kg · d) and intragastric administration of nicotinamide 100 mg/(kg · d) at d2 for continuous 28d .Group NC and group DC were given qod subcutaneous injection of isovol-ume normal saline ,and then intraperitoneal injection of isovolume 5% glucose and neutralized dose of insulin and intragastric administration of autoclaving water for continuous 4 weeks .Fasting blood glucose(FBG) ,fasting insu-lin ,number and affinity of insulin receptor on erythrocyte membrane in each group were measured after the experi-ment .[Results]After modeling ,FBG and insulin resistance(HOMA-IR) in group DC and group MI were signifi-cantly increased in comparison with group NC ( P < 0 .01) .Compared with group DC ,blood sugar in group MI was decreased by 33 .95% ,and HOMA-IR was decreased significantly( P<0 .05) .Compared with group NC ,the number of insulin receptor on erythrocyte membrane in group DC was decreased significantly ( P <0 .05) .Com-pared with group DC ,the number of insulin receptor on erythrocyte membrane in group MI was increased signifi-cantly( P <0 .05) .There was no significant difference in affinity of insulin receptor on erythrocyte membrane a-mong 3 groups( P>0 .05) .[Conclusion]The rh-CSF combined with hepatocyte growth-promoting factor and nico-tinamide can reduce the hyperglycemia of T 2DM in rats ,and improve insulin resistance .The mechanism may be associated with the increasing of the number of insulin receptor on cell membrane .