黑龙江医药
黑龍江醫藥
흑룡강의약
HEILONGJIANG MEDICAL JOURNAL
2014年
2期
304-305,306
,共3页
降糖宁%Rg1%Re%HPLC
降糖寧%Rg1%Re%HPLC
강당저%Rg1%Re%HPLC
Jiangtangning capsules%ginsenoside Rg1%ginsenoside Re%HPLC
目的：建立HPLC法测定降糖宁胶囊中人参含量的方法。方法：Packed ODS-A C18色谱柱(4.6 mm×250 mm，5μm)，以乙腈-0.05％磷酸溶液(100：400)为流动相；检测波长为203nm，流速为1ml/min。结果：人参皂苷Rg1、人参皂苷Re进样量均在0.4μg～1.6μg范围内进样量与峰面积呈良好线性关系(r=0.9996)；方法的平均回收率：人参皂苷Rg1为98.68%，RSD为1.71%(n=9)；人参皂苷Re为100.81%，2.71%(n=9)。结论：通过采用本方法对降糖宁胶囊人参中（Rg1、Re）的含量进行测定，方法准确可行，灵敏度高，能够有效控制产品质量。
目的：建立HPLC法測定降糖寧膠囊中人參含量的方法。方法：Packed ODS-A C18色譜柱(4.6 mm×250 mm，5μm)，以乙腈-0.05％燐痠溶液(100：400)為流動相；檢測波長為203nm，流速為1ml/min。結果：人參皂苷Rg1、人參皂苷Re進樣量均在0.4μg～1.6μg範圍內進樣量與峰麵積呈良好線性關繫(r=0.9996)；方法的平均迴收率：人參皂苷Rg1為98.68%，RSD為1.71%(n=9)；人參皂苷Re為100.81%，2.71%(n=9)。結論：通過採用本方法對降糖寧膠囊人參中（Rg1、Re）的含量進行測定，方法準確可行，靈敏度高，能夠有效控製產品質量。
목적：건립HPLC법측정강당저효낭중인삼함량적방법。방법：Packed ODS-A C18색보주(4.6 mm×250 mm，5μm)，이을정-0.05％린산용액(100：400)위류동상；검측파장위203nm，류속위1ml/min。결과：인삼조감Rg1、인삼조감Re진양량균재0.4μg～1.6μg범위내진양량여봉면적정량호선성관계(r=0.9996)；방법적평균회수솔：인삼조감Rg1위98.68%，RSD위1.71%(n=9)；인삼조감Re위100.81%，2.71%(n=9)。결론：통과채용본방법대강당저효낭인삼중（Rg1、Re）적함량진행측정，방법준학가행，령민도고，능구유효공제산품질량。
0bjective:To establish the determination methods of ginsenoside Rg1, ginsenoside Re In Jiangtangning capsules by HPLC. Methods:A Packed ODS-A Cl8 column(4.6mm × 250mm,5μm), Acetonitrile-0.05%phosphoric acid solution (100:400) as the mobile phase. The detection wavelength was 203nm.The flow rate was 1 ml/min. Results:The linear range of ginsenoside Rg1, ginsenoside Re were both 0.4~1.6μg (r=0.9996). The average recovery of the method:ginsenoside Rg1 was 98.68%, RSD was 171%(n=9);ginsenoside Re 100.81%, 2.71%(n=9). Conclusion:the content was determined by the method of ginsenoside Rg1, ginsenoside Re in Jiangtangning capsules, the methods are accurate, high sensitivity, and can effectively control the quality of the products.
